Anti-FH antibody [8F12BB5] (ab110286)

  Flow Cytometry - FH antibody [8F12BB5] (ab110286)

HeLa cells were stained with 1 µg/mL ab110286 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

  Immunocytochemistry/ Immunofluorescence - FH antibody [8F12BB5] (ab110286)

Immunocytochemistry image of FH (ab110286) stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110286 at 5µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.

  Immunoprecipitation - FH antibody [8F12BB5] (ab110286)

FH immunocaptured from HepG2 cells (Lane 1), human liver mitochondria (Lane 2), bovine liver mitochondria (Lane 3), and rat liver mitochondria (Lane 4) using ab110286.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FH antibody [8F12BB5] (ab110286)

ab110286 staining FH in formalin-fixed, paraffin-embedded human cerebellum tissue by Immunohistochemistry at 5ug/ml. FH immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.