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Western blot - FH antibody (ab62825)
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Product Name
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FH antibody
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See all FH antibodies (2)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to FH
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Immunogen
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Synthetic peptide: C-HPNDHVNKSQSSND, corresponding to internal sequence amino acids 176-189 of Human FH
BLAST 'C-HPNDHVNKSQSSND' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Many, C tra, Cow, CynMk, Dm, Hrs, Pig, Zfsh, Orngt
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/64000.
WB: Use at a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 55 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Kidney, Liver and Lung lysates
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Cellular localization
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Cytoplasmic and Mitochondrial
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Signal Transduction >> Metabolism >> Energy Metabolism
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Relevance
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FH (Fumarate hydratse) catalyzes the conversion of fumarate to malate. It is a key enzyme in the tricarboxylic acid (TCA) cycle. It exists in a cytosolic form and an N terminal extended form. The N terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. FH also acts as a tumor suppressor.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FH antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FH antibody images, references, products related to ab62825 and other tools.
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FH antibody images:
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References for FH antibody (ab62825)
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This product has been used in:
Kienhfer J et al. Association of mitochondrial antioxidant enzymes with mitochondrial DNA as integral nucleoid constituents. FASEB J 23:2034-44 (2009). WB; Human. PubMed: 19228881
If you publish research using ab62825 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to FH
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FH antibody - more information
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Customer reviews (feedback) regarding FH antibody |
Customer FAQs regarding FH antibody |
Protocols for FH antibody |
Price and availability of products related to FH antibody |
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FH antibody - related products:
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FH antibody - other tools:
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Contact Abcam with a Technical Enquiry about FH antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Western blot - FH antibody (ab62825)