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Western blot - FH antibody (ab62825)
Western blot - FH antibody (ab62825)
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Product Name 

FH antibody

 

See all FH antibodies (2)...

Product type 

Primary antibodies

Description 

Goat polyclonal to FH

Immunogen 

Synthetic peptide: C-HPNDHVNKSQSSND, corresponding to internal sequence amino acids 176-189 of Human FH
BLAST 'C-HPNDHVNKSQSSND' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat, Many, C tra, Cow, CynMk, Dm, Hrs, Pig, Zfsh, Orngt
(Due to sequence homology)

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: 1/64000.
WB: Use at a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 55 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human Kidney, Liver and Lung lysates

Cellular localization 

Cytoplasmic and Mitochondrial

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Signal Transduction >> Metabolism >> Energy Metabolism

Relevance 

FH (Fumarate hydratse) catalyzes the conversion of fumarate to malate. It is a key enzyme in the tricarboxylic acid (TCA) cycle. It exists in a cytosolic form and an N terminal extended form. The N terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. FH also acts as a tumor suppressor.

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

2271    (Human)

Omim

    

136850    (Human)

SwissProt

    

P07954    (Human)
P97807    (Mouse)

Unigene

    

592490    (Human)

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FH antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for FH antibody images, references, products related to ab62825 and other tools.

 

FH antibody images:

Product image  Western blot - FH antibody (ab62825)

Western blot - FH antibody (ab62825)

FH antibody (ab62825) at 0.01 µg/ml + Human Kidney lysate in RIPA buffer at 35 µg/ml

Predicted band size : 55 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)


Detected by chemiluminescence

 

References for FH antibody (ab62825)

Specific publication references   This product has been used in:

Kienhfer J et al. Association of mitochondrial antioxidant enzymes with mitochondrial DNA as integral nucleoid constituents. FASEB J 23:2034-44 (2009). WB; Human. PubMed: 19228881

If you publish research using ab62825 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to FH

 

FH antibody - more information

Customer reviews (feedback) regarding FH antibody

Customer FAQs regarding FH antibody

Protocols for FH antibody

Price and availability of products related to FH antibody

 

FH antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for FH antibody

ab30189

  

Kidney (Human) Cytoplasmic Lysate - adult normal tissue

 

Compatible Secondaries for FH antibody

ab6566

  

Cy5 ® antibody

ab6739

  

Texas Red ® antibody

ab6740

  

Biotin antibody

ab6741

  

HRP antibody

ab6742

  

Alkaline Phosphatase antibody

ab6881

  

FITC antibody

ab7004

  

Phycoerythrin antibody

ab27245

  

10nm Gold antibody

ab27246

  

10nm Gold antibody

ab27247

  

15nm Gold antibody

 

Other FH Antibodies

See all FH antibodies (2)...

 

FH antibody - other tools:

Contact Abcam with a Technical Enquiry about FH antibody

 

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