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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab53074? Please let us know so that we can cite the reference in this datasheet
ab53074 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Paraffin-embedded sections) - FHIT antibody (ab53074)
Immunohistochemical analysis of paraffin-embedded
human breast carcinoma tissue
using ab53074 at 1/50 dilution, with and without peptide immunogen.
Western blot - FHIT antibody (ab53074)
All lanes : Anti-FHIT antibody (ab53074) at 1/500 dilution
Lane 1 : A549 cell extract with no immunizing peptide
Lane 2 : A549 cell extract with immunizing peptide
Predicted band size : 17 kDa
Observed band size : 17 kDa
Immunocytochemistry/ Immunofluorescence-FHIT antibody(ab53074)
ICC/IF image of ab53074 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53074, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-FHIT antibody for Immunocytochemistry/ Immunofluorescence in Human (53074)
1
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