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Anti-FHIT antibody (ab53074)

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Overview

Product name

Anti-FHIT antibody
See all FHIT products (5) ...

Description

Rabbit polyclonal to FHIT

Specificity

FHIT antibody detects endogenous levels of total FHIT protein.

Tested applications

WB, IHC-P, ELISA, ICC/IFmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide derived from human FHIT

Positive control

Human breast carcinoma; A549 cell extract.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.

Clonality

Polyclonal

Isotype

IgG

  • Immunohistochemistry (Paraffin-embedded sections) - FHIT antibody (ab53074)Immunohistochemistry (Paraffin-embedded sections) - FHIT antibody (ab53074) image (enlarge)

  • Western blot - FHIT antibody (ab53074)Western blot - FHIT antibody (ab53074) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence-FHIT antibody(ab53074)Immunocytochemistry/ Immunofluorescence-FHIT antibody(ab53074) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab53074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

  • ELISA

     ELISA: 1/10000

    ELISA: 1/10000

  • ShowHide1 Image

    ICC/IF

     ICC/IF: Use a concentrat...Read more →

    ICC/IF: Use a concentration of 1 - 5 µg/ml.

Target

Relevance

FHIT (fragile histidine triad) cleaves adenosine 5' PPP 5' A to yield AMP and ADP. Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor. FHIT physically associates with ubiquitin conjugating enzyme 9.

Cellular localization

Cytoplasmic

Alternative names

  • AP3A hydrolase antibody
  • AP3A hydrolase fragile site 3p14.2 antibody
  • AP3Aase antibody
  • Bis 5' adenosyl triphosphatase antibody
  • Diadenosine 5' 5''' P1 P3 triphosphate hydrolase antibody
  • Dinucleosidetriphosphatase antibody
  • FRA 3B antibody
  • FRA3B antibody
  • Fragile histidine triad antibody
  • Fragile histidine triad gene antibody
  • Fragile histidine triad protein antibody
  • Tumor suppressor protein antibody
see all

Anti-FHIT antibody images:

  Immunohistochemistry (Paraffin-embedded sections) - FHIT antibody (ab53074)

Immunohistochemistry (Paraffin-embedded sections) - FHIT antibody (ab53074)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue
using ab53074 at 1/50 dilution, with and without peptide immunogen.

  Western blot - FHIT antibody (ab53074)

Western blot - FHIT antibody (ab53074)

All lanes : Anti-FHIT antibody (ab53074) at 1/500 dilution

Lane 1 : A549 cell extract with no immunizing peptide
Lane 2 : A549 cell extract with immunizing peptide


Predicted band size : 17 kDa
Observed band size : 17 kDa

  Immunocytochemistry/ Immunofluorescence-FHIT antibody(ab53074)

Immunocytochemistry/ Immunofluorescence-FHIT antibody(ab53074)

ICC/IF image of ab53074 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53074, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-FHIT antibody (ab53074)

ab53074 has not yet been referenced specifically in any publications.

Publishing research using ab53074? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"