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Products:Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Overview
Product name
Anti-FHIT antibody
See all FHIT products (5) ...
Description
Rabbit polyclonal to FHIT
Specificity
FHIT antibody detects endogenous levels of total FHIT protein.
Tested applications
WB, IHC-P, ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide derived from human FHIT
Positive control
Human breast carcinoma; A549 cell extract.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/10000ELISA: 1/10000
ICC/IF: Use a concentration of 1 - 5 µg/ml.
Target
Relevance
FHIT (fragile histidine triad) cleaves adenosine 5' PPP 5' A to yield AMP and ADP. Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor. FHIT physically associates with ubiquitin conjugating enzyme 9.
Cellular localization
Cytoplasmic
Alternative names
- AP3A hydrolase antibody
- AP3A hydrolase fragile site 3p14.2 antibody
- AP3Aase antibody
see all
Database links
- Entrez Gene: 2272 Human
- Omim: 601153 Human
- SwissProt: P49789 Human
- Unigene: 655995 Human
Anti-FHIT antibody images:
Immunohistochemistry (Paraffin-embedded sections) - FHIT antibody (ab53074)
Immunohistochemical analysis of paraffin-embedded
human breast carcinoma tissue
using ab53074 at 1/50 dilution, with and without peptide immunogen.
Western blot - FHIT antibody (ab53074)
All lanes : Anti-FHIT antibody (ab53074) at 1/500 dilution
Lane 1 : A549 cell extract with no immunizing peptide
Lane 2 : A549 cell extract with immunizing peptide
Predicted band size : 17 kDa
Observed band size : 17 kDa
Immunocytochemistry/ Immunofluorescence-FHIT antibody(ab53074)
ICC/IF image of ab53074 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53074, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-FHIT antibody (ab53074)
ab53074 has not yet been referenced specifically in any publications.
Publishing research using ab53074? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"