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Immunohistochemistry (Paraffin-embedded sections) - FHIT antibody (ab53074)
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Western blot - FHIT antibody (ab53074)
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Immunocytochemistry/ Immunofluorescence-FHIT antibody(ab53074)
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Product Name
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FHIT antibody
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See all FHIT antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to FHIT
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Immunogen
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Synthetic peptide derived from human FHIT
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Reacts with
(species key)
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Hu
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Specificity
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FHIT antibody detects endogenous levels of total FHIT protein.
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
ICC/IF: Use at a concentration of 1 - 5 µg/ml.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human breast carcinoma; A549 cell extract.
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Cellular localization
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Cytoplasmic
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
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Relevance
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FHIT (fragile histidine triad) cleaves adenosine 5' PPP 5' A to yield AMP and ADP. Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor. FHIT physically associates with ubiquitin conjugating enzyme 9.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FHIT antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FHIT antibody images, references, products related to ab53074 and other tools.
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FHIT antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - FHIT antibody (ab53074)
Immunohistochemical analysis of paraffin-embedded
human breast carcinoma tissue
using ab53074 at 1/50 dilution, with and without peptide immunogen.
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Western blot - FHIT antibody (ab53074)
All lanes : FHIT antibody (ab53074) at 1/500 dilution
Lane 1 : A549 cell extract with no immunizing peptide
Lane 2 : A549 cell extract with immunizing peptide
Predicted band size : 17 kDa
Observed band size : 17 kDa
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Immunocytochemistry/ Immunofluorescence-FHIT antibody(ab53074)
ICC/IF image of ab53074 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53074, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to FHIT
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FHIT antibody - more information
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Customer reviews (feedback) regarding FHIT antibody |
Customer FAQs regarding FHIT antibody |
Protocols for FHIT antibody |
Price and availability of products related to FHIT antibody |
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FHIT antibody - related products:
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FHIT antibody - other tools:
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Contact Abcam with a Technical Enquiry about FHIT antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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