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Immunohistochemistry (Paraffin-embedded sections) - FHIT antibody (ab53074)
Immunohistochemistry (Paraffin-embedded sections) - FHIT antibody (ab53074)
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Western blot - FHIT antibody (ab53074)
Western blot - FHIT antibody (ab53074)
(enlarge)


Immunocytochemistry/ Immunofluorescence-FHIT antibody(ab53074)
Immunocytochemistry/ Immunofluorescence-FHIT antibody(ab53074)
(enlarge)


Product Name 

FHIT antibody

 

See all FHIT antibodies (5)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to FHIT

Immunogen 

Synthetic peptide derived from human FHIT

Reacts with 
(species key)

Hu

Specificity 

FHIT antibody detects endogenous levels of total FHIT protein.

Tested applications 
(see key)

ELISA, ICC/IF, IHC-P, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: 1/10000.
ICC/IF: Use at a concentration of 1 - 5 µg/ml.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human breast carcinoma; A549 cell extract.

Cellular localization 

Cytoplasmic

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors

Relevance 

FHIT (fragile histidine triad) cleaves adenosine 5' PPP 5' A to yield AMP and ADP. Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor. FHIT physically associates with ubiquitin conjugating enzyme 9.

 

FHIT antibody (ab53074)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

2272    (Human)

Omim

    

601153    (Human)

SwissProt

    

P49789    (Human)

Unigene

    

655995    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FHIT antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for FHIT antibody images, references, products related to ab53074 and other tools.

 

FHIT antibody images:

Product image  Immunohistochemistry (Paraffin-embedded sections) - FHIT antibody (ab53074)

Immunohistochemistry (Paraffin-embedded sections) - FHIT antibody (ab53074)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue
using ab53074 at 1/50 dilution, with and without peptide immunogen.

 

Product image  Western blot - FHIT antibody (ab53074)

Western blot - FHIT antibody (ab53074)

All lanes : FHIT antibody (ab53074) at 1/500 dilution

Lane 1 : A549 cell extract with no immunizing peptide
Lane 2 : A549 cell extract with immunizing peptide


Predicted band size : 17 kDa
Observed band size : 17 kDa

 

Product image  Immunocytochemistry/ Immunofluorescence-FHIT antibody(ab53074)

Immunocytochemistry/ Immunofluorescence-FHIT antibody(ab53074)

ICC/IF image of ab53074 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53074, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

 
 

Search PubMed (MEDLINE) for references to FHIT

 

FHIT antibody - more information

Customer reviews (feedback) regarding FHIT antibody

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Price and availability of products related to FHIT antibody

 

FHIT antibody - related products:

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