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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> LIM
Overview
Product name
Anti-FHL2 antibody
See all FHL2 products (6) ...
Description
Rabbit polyclonal to FHL2
Specificity
Reactivity with mouse FHL2 is unlikely owing to divergence of this ortholog from the peptide used for antibody production. The epitope recognized by ab1237 maps to a region between residues 175 and 225 of human Four and a Half LIM domains 2 using the numbering given in entry NP_963849.1 (GeneID 2274).
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
A synthetic peptide which represented a portion of human FHL2 encoded within exon 7.
Positive control
HeLa nuclear extract.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody is affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> LIM
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/10,000.Detects a band of approximately 32 kDa (predicted molecular weight: 44.6 kDa).Can be blocked with FHL2 peptide (ab119360).
ICC/IF: 1/100
Target
Function
May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth.
Tissue specificity
Expressed in skeletal muscle and heart.
Sequence similarities
Contains 4 LIM zinc-binding domains.
Domain
The third LIM zinc-binding mediates interaction with E4F1.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ14192
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AAG11 antibody
- FHL 2 antibody
- LIM domain protein DRAL antibody
see all
Database links
- Entrez Gene: 2274 Human
- Entrez Gene: 14200 Mouse
- Omim: 602633 Human
- SwissProt: Q14192 Human
- SwissProt: O70433 Mouse
- SwissProt: Q8VDP9 Mouse
- Unigene: 443687 Human
- Unigene: 8302 Human
see all
Anti-FHL2 antibody images:
Western blot - FHL2 antibody (ab12327)
Predicted band size : 44.6 kDa
Samples: Nuclear extract (NE) from HeLa cells. Antibody: Affinity purified rabbit anti-FHL2 antibody ab12327 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 5 minutes.
Immunocytochemistry/ Immunofluorescence - FHL2 antibody (ab12327)
ICC/IF image of ab12327 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12327, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-FHL2 antibody (ab12327)
This product has been referenced in:
- Gökmen-Polar Yet al. Dual targeting of EphA2 and ER restores tamoxifen sensitivity in ER/EphA2-positive breast cancer. Breast Cancer Res Treat 127:375-84 (2011). WB; Human.Read more (PubMed: 20602165) »
- Mille Fet al. The Patched dependence receptor triggers apoptosis through a DRAL-caspase-9 complex. Nat Cell Biol 11:739-46 (2009). WB, ICC/IF; Human.Read more (PubMed: 19465923) »
See all 3 publications for this product
Publishing research using ab12327? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"