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Western blot - FHL2 antibody (ab12327)
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Immunocytochemistry/ Immunofluorescence-FHL2 antibody(ab12327)
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Product Name
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FHL2 antibody
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See all FHL2 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to FHL2
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Immunogen
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A synthetic peptide which represented a portion of human FHL2 encoded within exon 7.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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Reactivity with mouse FHL2 is unlikely owing to divergence of this ortholog from the peptide used for antibody production. The epitope recognized by ab1237 maps to a region between residues 175 and 225 of human Four and a Half LIM domains 2 using the numbering given in entry NP_963849.1 (GeneID 2274).
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/100.
WB: 1/1000 - 1/10,000. Detects a band of approximately 32 kDa (predicted molecular weight: 44.6 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa nuclear extract.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> LIM
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Relevance
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FHL2 is a LIM protein, all of which contain a highly conserved double zinc finger motif called the LIM domain. It is thought to be a co-activator of the androgen receptor and a transcription coactivator. FHL2 might enforce beta-catenin transactivation activity in cancer cells and may be downregulated during transformation of a variety of cell types.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This antibody is affinity purified.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FHL2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FHL2 antibody images, references, products related to ab12327 and other tools.
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FHL2 antibody images:
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 Western blot - FHL2 antibody (ab12327)
Predicted band size : 44.6 kDa
Samples: Nuclear extract (NE) from HeLa cells. Antibody: Affinity purified rabbit anti-FHL2 antibody ab12327 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 5 minutes.
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Immunocytochemistry/ Immunofluorescence-FHL2 antibody(ab12327)
ICC/IF image of ab12327 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12327, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for FHL2 antibody (ab12327)
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This product has been used in: (two most recent references)
Mille F et al. The Patched dependence receptor triggers apoptosis through a DRAL-caspase-9 complex. Nat Cell Biol 11:739-46 (2009). WB, ICC/IF; Human. PubMed: 19465923
Hamidouche Z et al. FHL2 mediates dexamethasone-induced mesenchymal cell differentiation into osteoblasts by activating Wnt/{beta}-catenin signaling-dependent Runx2 expression. FASEB J : (2008). PubMed: 18653765
If you publish research using ab12327 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to FHL2
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FHL2 antibody - more information
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Customer reviews (feedback) regarding FHL2 antibody |
Customer FAQs regarding FHL2 antibody |
Protocols for FHL2 antibody |
Price and availability of products related to FHL2 antibody |
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FHL2 antibody - related products:
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FHL2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about FHL2 antibody |
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