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Western blot - FHL2 antibody (ab12327)
Western blot - FHL2 antibody (ab12327)
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Immunocytochemistry/ Immunofluorescence-FHL2 antibody(ab12327)
Immunocytochemistry/ Immunofluorescence-FHL2 antibody(ab12327)
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Product Name 

FHL2 antibody

 

See all FHL2 antibodies (6)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to FHL2

Immunogen 

A synthetic peptide which represented a portion of human FHL2 encoded within exon 7.

Reacts with 
(species key)

Hu, Ms

Specificity 

Reactivity with mouse FHL2 is unlikely owing to divergence of this ortholog from the peptide used for antibody production. The epitope recognized by ab1237 maps to a region between residues 175 and 225 of human Four and a Half LIM domains 2 using the numbering given in entry NP_963849.1 (GeneID 2274).

Tested applications 
(see key)

ICC/IF, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/100.
WB: 1/1000 - 1/10,000. Detects a band of approximately 32 kDa (predicted molecular weight: 44.6 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa nuclear extract.

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> LIM

Relevance 

FHL2 is a LIM protein, all of which contain a highly conserved double zinc finger motif called the LIM domain. It is thought to be a co-activator of the androgen receptor and a transcription coactivator. FHL2 might enforce beta-catenin transactivation activity in cancer cells and may be downregulated during transformation of a variety of cell types.

 

FHL2 antibody (ab12327)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

2274    (Human)
14200    (Mouse)

Omim

    

602633    (Human)

SwissProt

    

Q14192    (Human)
O70433    (Mouse)
Q8VDP9    (Mouse)

Unigene

    

8302    (Human)
6799    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This antibody is affinity purified.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FHL2 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for FHL2 antibody images, references, products related to ab12327 and other tools.

 

FHL2 antibody images:

Product image  Western blot - FHL2 antibody (ab12327)

Western blot - FHL2 antibody (ab12327)



Predicted band size : 44.6 kDa


Samples: Nuclear extract (NE) from HeLa cells. Antibody: Affinity purified rabbit anti-FHL2 antibody ab12327 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 5 minutes.

 

Product image  Immunocytochemistry/ Immunofluorescence-FHL2 antibody(ab12327)

Immunocytochemistry/ Immunofluorescence-FHL2 antibody(ab12327)

ICC/IF image of ab12327 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12327, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

 

References for FHL2 antibody (ab12327)

Specific publication references   This product has been used in: (two most recent references)

Mille F et al. The Patched dependence receptor triggers apoptosis through a DRAL-caspase-9 complex. Nat Cell Biol 11:739-46 (2009). WB, ICC/IF; Human. PubMed: 19465923

Hamidouche Z et al. FHL2 mediates dexamethasone-induced mesenchymal cell differentiation into osteoblasts by activating Wnt/{beta}-catenin signaling-dependent Runx2 expression. FASEB J : (2008). PubMed: 18653765
If you publish research using ab12327 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to FHL2

 

FHL2 antibody - more information

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Other FHL2 Antibodies

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