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Anti-FHL2 antibody (ab12327)

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Overview

Product name

Anti-FHL2 antibody
See all FHL2 products (6) ...

Description

Rabbit polyclonal to FHL2

Specificity

Reactivity with mouse FHL2 is unlikely owing to divergence of this ortholog from the peptide used for antibody production. The epitope recognized by ab1237 maps to a region between residues 175 and 225 of human Four and a Half LIM domains 2 using the numbering given in entry NP_963849.1 (GeneID 2274).

Tested applications

WB, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

A synthetic peptide which represented a portion of human FHL2 encoded within exon 7.

Positive control

HeLa nuclear extract.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

This antibody is affinity purified.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - FHL2 antibody (ab12327)Western blot - FHL2 antibody (ab12327) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - FHL2 antibody (ab12327)Immunocytochemistry/ Immunofluorescence - FHL2 antibody (ab12327) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab12327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth.

Tissue specificity

Expressed in skeletal muscle and heart.

Sequence similarities

Contains 4 LIM zinc-binding domains.

Domain

The third LIM zinc-binding mediates interaction with E4F1.

Cellular localization

Cytoplasm. Nucleus.

Target information above from: UniProt accessionQ14192 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • AAG11 antibody
  • FHL 2 antibody
  • LIM domain protein DRAL antibody
  • AAG 11 antibody
  • AAG11 antibody
  • Aging associated gene 11 antibody
  • Downregulated in rhabdomyosarcoma LIM protein antibody
  • DRAL antibody
  • FHL 2 antibody
  • FHL-2 antibody
  • Fhl2 antibody
  • FHL2 protein antibody
  • FHL2_HUMAN antibody
  • Four and a half LIM domain protein 2 antibody
  • Four and a half LIM domains 2 antibody
  • Four and a half LIM domains protein 2 antibody
  • LIM domain protein DRAL antibody
  • Skeletal muscle LIM protein 3 antibody
  • Skeletal muscle LIM-protein 3 antibody
  • SLIM 3 antibody
  • SLIM-3 antibody
  • SLIM3 antibody
see all

Anti-FHL2 antibody images:

  Western blot - FHL2 antibody (ab12327)

Western blot - FHL2 antibody (ab12327)



Predicted band size : 44.6 kDa


Samples: Nuclear extract (NE) from HeLa cells. Antibody: Affinity purified rabbit anti-FHL2 antibody ab12327 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 5 minutes.

  Immunocytochemistry/ Immunofluorescence - FHL2 antibody (ab12327)

Immunocytochemistry/ Immunofluorescence - FHL2 antibody (ab12327)

ICC/IF image of ab12327 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12327, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-FHL2 antibody (ab12327)

This product has been referenced in:

  • Gökmen-Polar Yet al. Dual targeting of EphA2 and ER restores tamoxifen sensitivity in ER/EphA2-positive breast cancer. Breast Cancer Res Treat 127:375-84 (2011). WB; Human.Read more (PubMed: 20602165) »
  • Mille Fet al. The Patched dependence receptor triggers apoptosis through a DRAL-caspase-9 complex. Nat Cell Biol 11:739-46 (2009). WB, ICC/IF; Human.Read more (PubMed: 19465923) »

See all 3 publications for this product

Publishing research using ab12327? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"