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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - FHL2 antibody (ab12328)
Predicted band size : 44.6 kDa
Samples: Nuclear extract (NE) from HeLa cells. Antibody: Affinity purified rabbit anti-FHL2 antibody ab12328 used at 0.3 mg/ml. Detection: Chemiluminescence with an exposure time of 5 minutes.
Immunocytochemistry/ Immunofluorescence-FHL2 antibody(ab12328)
ICC/IF image of ab12328 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12328, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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