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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> LIM
Overview
Product name
Anti-FHL2 antibody
See all FHL2 products (6) ...
Description
Rabbit polyclonal to FHL2
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
A synthetic peptide which represented a portion of human FHL2 encoded within exon 8.
Positive control
HeLa nuclear extract.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody is affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> LIM
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/1000 - 1/10,000. Detects a band of approximately 32 kDa, processed product (predicted molecular weight: 44.6 kDa, unprocessed precursot).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth.
Tissue specificity
Expressed in skeletal muscle and heart.
Sequence similarities
Contains 4 LIM zinc-binding domains.
Domain
The third LIM zinc-binding mediates interaction with E4F1.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ14192
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AAG11 antibody
- FHL 2 antibody
- LIM domain protein DRAL antibody
see all
Database links
- Entrez Gene: 2274 Human
- Entrez Gene: 14200 Mouse
- Omim: 602633 Human
- SwissProt: Q14192 Human
- SwissProt: O70433 Mouse
- SwissProt: Q8VDP9 Mouse
- Unigene: 443687 Human
- Unigene: 8302 Human
see all
Anti-FHL2 antibody images:
Western blot - FHL2 antibody (ab12328)
Predicted band size : 44.6 kDa
Samples: Nuclear extract (NE) from HeLa cells. Antibody: Affinity purified rabbit anti-FHL2 antibody ab12328 used at 0.3 mg/ml. Detection: Chemiluminescence with an exposure time of 5 minutes.
Immunocytochemistry/ Immunofluorescence-FHL2 antibody(ab12328)
ICC/IF image of ab12328 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12328, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-FHL2 antibody (ab12328)
ab12328 has not yet been referenced specifically in any publications.
Publishing research using ab12328? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"