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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> LIM
Overview
Product name
Anti-FHL2 antibody
See all FHL2 products (6) ...
Description
Goat polyclonal to FHL2
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Fruit fly (Drosophila melanogaster), Zebrafish
Immunogen
Synthetic peptide: RDDILCPDCGKDI, corresponding to C terminal amino acids 267-279 of FHL2.
RDDILCPDCG KDI
Epitope
C Terminus
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by immunogen affinity chromatography.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> LIM
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Western blot - FHL2 antibody (ab15715)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab15715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: antibody detection limit dilution 1/16000.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 32 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth.
Tissue specificity
Expressed in skeletal muscle and heart.
Sequence similarities
Contains 4 LIM zinc-binding domains.
Domain
The third LIM zinc-binding mediates interaction with E4F1.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ14192
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AAG11 antibody
- FHL 2 antibody
- LIM domain protein DRAL antibody
see all
Database links
- Entrez Gene: 2274 Human
- Entrez Gene: 14200 Mouse
- Entrez Gene: 63839 Rat
- Omim: 602633 Human
- SwissProt: Q14192 Human
- SwissProt: O70433 Mouse
- SwissProt: Q8VDP9 Mouse
- SwissProt: O35115 Rat
see all
Anti-FHL2 antibody images:
Western blot - FHL2 antibody (ab15715)
Anti-FHL2 antibody (ab15715) at 1 µg/ml + Tissue lysates prepared from human heart at 35 µg
developed using the ECL technique
Predicted band size : 32 kDa
Primary antibody was incubated for 1 hour.
References for Anti-FHL2 antibody (ab15715)
ab15715 has not yet been referenced specifically in any publications.
Publishing research using ab15715? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"