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Anti-FHL2 antibody (ab15715)

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Overview

Product name

Anti-FHL2 antibody
See all FHL2 products (6) ...

Description

Goat polyclonal to FHL2

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Fruit fly (Drosophila melanogaster), Zebrafish

Immunogen

Synthetic peptide: RDDILCPDCGKDI, corresponding to C terminal amino acids 267-279 of FHL2.

RDDILCPDCG KDI

Epitope

C Terminus

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA. pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by immunogen affinity chromatography.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - FHL2 antibody (ab15715)Western blot - FHL2 antibody (ab15715) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab15715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: antibody detection limit dilution 1/16000.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 32 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth.

Tissue specificity

Expressed in skeletal muscle and heart.

Sequence similarities

Contains 4 LIM zinc-binding domains.

Domain

The third LIM zinc-binding mediates interaction with E4F1.

Cellular localization

Cytoplasm. Nucleus.

Target information above from: UniProt accessionQ14192 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • AAG11 antibody
  • FHL 2 antibody
  • LIM domain protein DRAL antibody
  • AAG 11 antibody
  • AAG11 antibody
  • Aging associated gene 11 antibody
  • Downregulated in rhabdomyosarcoma LIM protein antibody
  • Downregulated in rhabdomyosarcoma LIM protein antibody
  • DRAL antibody
  • DRAL antibody
  • FHL 2 antibody
  • FHL-2 antibody
  • Fhl2 antibody
  • FHL2 protein antibody
  • FHL2_HUMAN antibody
  • Four and a half LIM domain protein 2 antibody
  • Four and a half LIM domains 2 antibody
  • Four and a half LIM domains protein 2 antibody
  • Four and a half LIM domains protein 2 antibody
  • LIM domain protein DRAL antibody
  • Skeletal muscle LIM protein 3 antibody
  • Skeletal muscle LIM protein 3 antibody
  • Skeletal muscle LIM-protein 3 antibody
  • SLIM 3 antibody
  • SLIM-3 antibody
  • SLIM3 antibody
  • SLIM3 antibody
see all

Anti-FHL2 antibody images:

  Western blot - FHL2 antibody (ab15715)

Western blot - FHL2 antibody (ab15715)

Anti-FHL2 antibody (ab15715) at 1 µg/ml + Tissue lysates prepared from human heart at 35 µg
developed using the ECL technique

Predicted band size : 32 kDa


Primary antibody was incubated for 1 hour.

References for Anti-FHL2 antibody (ab15715)

ab15715 has not yet been referenced specifically in any publications.

Publishing research using ab15715? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"