|
|
|
|
Western blot - FHL2 antibody (ab15715)

(enlarge)
|
|
|
|
|
Product Name
|
FHL2 antibody
|
| |
See all FHL2 antibodies (6)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Goat polyclonal to FHL2
|
|
Immunogen
|
Synthetic peptide: RDDILCPDCGKDI, corresponding to C terminal amino acids 267-279 of FHL2. BLAST 'RDDILCPDCGKDI' with or 
|
|
Reacts with
|
Hu
|
|
Predicted to react
(species key)
|
Ms, Rat, Zfsh (Due to sequence homology)
|
|
Epitope
|
C Terminus
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions ELISA: antibody detection limit dilution 1/16000. WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 32 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Cytoplasmic and Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> LIM
|
|
Relevance
|
FHL2 is a LIM protein, all of which contain a highly conserved double zinc finger motif called the LIM domain. It is thought to be a co-activator of the androgen receptor and a transcription coactivator. FHL2 might enforce beta-catenin transactivation activity in cancer cells and may be downregulated during transformation of a variety of cell types.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window
|
|
Raised in
|
Goat
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide Constituents: 0.5% BSA. pH 7.3 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
|
|
Purification notes
|
Purified from goat serum by ammonium sulphate precipitation followed by immunogen affinity chromatography.
|
|
Form
|
Liquid
|
|
Concentration
|
0.500 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FHL2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for FHL2 antibody images, references, products related to ab15715 and other tools.
|
| |
FHL2 antibody images:
|
Western blot - FHL2 antibody (ab15715)
FHL2 antibody (ab15715) at 1 µg/ml + Tissue lysates prepared from human heart at 35 µg developed using the ECL technique
Predicted band size : 32 kDa
Primary antibody was incubated for 1 hour.
|
| |
| |
|
Search PubMed (MEDLINE) for references to FHL2
|
| |
FHL2 antibody - more information
|
Customer reviews (feedback) regarding FHL2 antibody |
Customer FAQs regarding FHL2 antibody |
Protocols for FHL2 antibody |
Price and availability of products related to FHL2 antibody |
| |
FHL2 antibody - related products:
|
|
|
| |
FHL2 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about FHL2 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"