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- Secondary antibodies
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Product name
Anti-FHL2 antibody
See all FHL2 products (6) ...
Description
Rabbit polyclonal to FHL2
Specificity
ab56068 detects FHL2. It also binds FHL2 isoform 5 with MW of 389 amino acids (~42kDa).
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide derived from the human FHL2 protein
Positive control
Antibody tested on HeLa Lysates
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.08% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purification notes
Purified by SAS precipitation.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> LIM
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Western blot - FHL2 antibody (ab56068)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa).
Target
Function
May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth.
Tissue specificity
Expressed in skeletal muscle and heart.
Sequence similarities
Contains 4 LIM zinc-binding domains.
Domain
The third LIM zinc-binding mediates interaction with E4F1.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ14192
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AAG11 antibody
- FHL 2 antibody
- LIM domain protein DRAL antibody
see all
Database links
- Entrez Gene: 2274 Human
- Omim: 602633 Human
- SwissProt: Q14192 Human
- Unigene: 8302 Human
- Unigene: 443687 Human
Anti-FHL2 antibody images:
Western blot - FHL2 antibody (ab56068)
Lanes 1 - 2 : Anti-FHL2 antibody (ab56068) at 0.4 µg/ml
Lane 3 :
Lane 1 : HeLa cell extract at 10ug/lane
Lane 2 : HeLa cell extract at 10ug/lane with 16 ug blocking peptide
Lane 3 : HeLa cell extract at 10ug/lane. Goat anti Rabbit HRP control
Secondary
goat anti-rabbit Ig HRP at 1/75000 dilution
developed using the ECL technique
Predicted band size : 32 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
References for Anti-FHL2 antibody (ab56068)
ab56068 has not yet been referenced specifically in any publications.
Publishing research using ab56068? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"