Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> LIM
Overview
Product name
Anti-FHL2 antibody
See all FHL2 products (6) ...
Description
Rabbit polyclonal to FHL2
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog, Zebrafish
Immunogen
Synthetic peptide: YAKKCAGCTN PISGLGGTKY ISFEERQWHN DCFNCKKCSL SLVGRGFLTE, corresponding to a region within C terminal amino acids 217-266 of Human FHL2
YAKKCAGCTN PISGLGGTKY ISFEERQWHN DCFNCKKCSL SLVGRGFLTE
Positive control
Myocardium
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> LIM
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.6 µg/mlDetects a band of approximately 30 kDa (predicted molecular weight: 32 kDa).
IHC-P: Use a concentration of 4 - 8 µg/ml.
Target
Function
May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth.
Tissue specificity
Expressed in skeletal muscle and heart.
Sequence similarities
Contains 4 LIM zinc-binding domains.
Domain
The third LIM zinc-binding mediates interaction with E4F1.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ14192
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AAG11 antibody
- FHL 2 antibody
- LIM domain protein DRAL antibody
see all
Database links
- Entrez Gene: 2274 Human
- Entrez Gene: 14200 Mouse
- Entrez Gene: 63839 Rat
- Omim: 602633 Human
- SwissProt: Q14192 Human
- SwissProt: O70433 Mouse
- SwissProt: Q8VDP9 Mouse
- SwissProt: O35115 Rat
see all
Anti-FHL2 antibody images:
Western blot - FHL2 antibody (ab66399)
Anti-FHL2 antibody (ab66399) at 0.6 µg/ml + Fetal heart lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 32 kDa
Observed band size : 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FHL2 antibody (ab66399)
ab66399, at 8µg/ml, staining Human FHL2 in myocardial cells (indicated with arrows) in paraffin-embedded heart tissue, by Immunohistochemistry, 400x magnification.
References for Anti-FHL2 antibody (ab66399)
This product has been referenced in:
- Isumi Yet al. Transgenic overexpression of USP15 in the heart induces cardiac remodeling in mice. Biochem Biophys Res Commun 405:216-21 (2011). WB; Human.Read more (PubMed: 21219870) »
See 1 publication for this product
Publishing research using ab66399? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"