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FITC Protein Blocking Agent (PBA) (ab128980)

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Overview

  • Product nameFITC Protein Blocking Agent (PBA)
  • Tested applicationsICC/IF, IHC-Fr, IHC-P more details
  • General notesBlocks nonspecific antibody (protein) binding of primary reagent.

    Reagent: 6 ml

    Procedure:

    FOR USE IN FLUORESCENCE MICROSCOPY

    1: After slides are hydrated to water, wash slides in buffer solution for 2 minutes.

    2: Remove slide from buffer wash, wipe around tissue and apply sufficient drops of FITC Protein Blocking Agent (PBA) to cover the entire section..

    3: Incubate for 5 to 20 minutes at room temperature..

    4: DO NOT WASH TISSUE SECTIONS IN BUFFER. Carefully tap off excess PBA, wipe around tissue and remove the residual PBA to prevent further dilution of subsequent primary reagent application..

    5: Apply the appropriate primary antibody/ primary reagent to cover the entire section and proceed with immunostaining protocol.

    These directions are recommendations; however, they may be altered according to procedures established in-house.).

    IHC Protocol Summary

    • Properties

  • Storage instructionsStore at +4°C in the dark. Do not freeze.
  • Storage bufferPreservative: 0.1% Sodium azide
  • Concentration information loading...
  • Research Areas
    • Alternative names
        FITC protein blockProtein block

    Applications

    Our Abpromise guarantee covers the use of ab128980 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ICC/IF ICC/IF: Use at an assay dependent concentration.
    IHC-Fr IHC-Fr: Use at an assay dependent concentration.
    IHC-P IHC-P: Use at an assay dependent concentration.

    References for FITC Protein Blocking Agent (PBA) (ab128980)

    ab128980 has not yet been referenced specifically in any publications.

    Publishing research using ab128980 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"