FITC Protein Blocking Agent (PBA) (ab128980)
Reagent: 6 ml
FOR USE IN FLUORESCENCE MICROSCOPY
1: After slides are hydrated to water, wash slides in buffer solution for 2 minutes.
2: Remove slide from buffer wash, wipe around tissue and apply sufficient drops of FITC Protein Blocking Agent (PBA) to cover the entire section..
3: Incubate for 5 to 20 minutes at room temperature..
4: DO NOT WASH TISSUE SECTIONS IN BUFFER. Carefully tap off excess PBA, wipe around tissue and remove the residual PBA to prevent further dilution of subsequent primary reagent application..
5: Apply the appropriate primary antibody/ primary reagent to cover the entire section and proceed with immunostaining protocol.
These directions are recommendations; however, they may be altered according to procedures established in-house.).
- FITC protein blockProtein block
Our Abpromise guarantee covers the use of ab128980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: Use at an assay dependent concentration.|
|IHC-Fr||IHC-Fr: Use at an assay dependent concentration.|
|IHC-P||IHC-P: Use at an assay dependent concentration.|
References for FITC Protein Blocking Agent (PBA) (ab128980)
ab128980 has not yet been referenced specifically in any publications.