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Anti-FITC antibody [2A3] (ab10257)

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Overview

  • Product nameAnti-FITC antibody [2A3]See all FITC primary antibodies ...
  • Description
    Mouse monoclonal [2A3] to FITC
  • Specificityab10257 reacts with both free and conjugated FITC.
  • Tested applicationsIHC-P, IHC-Fr more details
  • Immunogen

    FITC-BSA conjugate.

  • General notesConcentration varies from lot to lot and can be provided on request.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurity tested by electrophoresis.
  • Clonality Monoclonal
  • Clone number2A3
  • MyelomaSp2/0
  • IsotypeIgG2a
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab10257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P
IHC-Fr
  • Application notesIHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.

    Detection of FITC-labeled antigens or antibodies.
    Capture antibody for FITC-labeled biopolymers.
    Enzyme-labeled second antibody for immunohistochemistry with FITC-conjugated mouse monoclonal antibodies.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • RelevanceFITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
    • Alternative names
        Fluorescein Isothiocyanate antibody

    References for Anti-FITC antibody [2A3] (ab10257)

    ab10257 has not yet been referenced specifically in any publications.

    Publishing research using ab10257 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab10257 Submit a Question for ab10257

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    Hi, can you tell me how much you have left of the following antibody lot:   Mouse anti-fluorescein antibody: cat # ab10257, lot # GR4859-5  

    Thank you for contacting us. We have no vials of GR4859-5 but we do have six vials of GR4859-6, which is the same stock as GR4859-5, but aliquotted on a different date. Please do not hesitate to contact us if you need any more advice or inf...

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    Can this antibody also recognize and bind other fluorescein derivatives, e.g. FAM?

    Unfortunately ab10257 has not been tested to react with FAM. I am sorry for not being more helpful on this occasion.

    Has this antibody been tested in IHC on frozen or paraffin-embedded sections?

    Thank you for your enquiry. Ab10257 can be used in IHC in two ways: 1. You can use FITC-labeled antibody against antigen of your interest as first antibody and then apply peroxidase-labeled ab10257 as developing reagent. 2. You can add the excess o...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"