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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab24373 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - FKBP12 antibody (ab24373)
All lanes : Anti-FKBP12 antibody (ab24373) at 1 µg/ml
Lane 1 : Human Brain Tissue Lysate
Lane 2 : Mouse Brain Tissue Lysate
Lane 3 : Rat Brain Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 12 kDa
Observed band size : 12 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FKBP12 antibody (ab24373)
ab24373 staining FKBP12 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed in formaldehyde before heat mediated antigen retrieval and then blocked for 45 minutes at 20°C (5 minutes of peroxidase block and 10 minutes of protein block). The primary antibody was diluted, 1/2000 in PBS and incubated with sample for 45 minutes at 21°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
Western blot - Anti-FKBP12 antibody (ab24373)
Anti-FKBP12 antibody (ab24373) at 1 µg/ml +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 2 minutes
FKBP12 antibody for WB in Rat (24373)
FKBP12 antibody for WB in Mouse (24373)
FKBP12 antibody for IHC-P in Human (24373)
FKBP12 antibody for IHC-P in Mouse (24373)
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