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Western blot - FKBP12 antibody (ab24373)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FKBP12 antibody (ab24373)
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Product Name
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FKBP12 antibody
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See all FKBP12 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to FKBP12
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FKBP12.
(Peptide available as ab25840.)
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Reacts with
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Hu, Ms, Rat
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Predicted to react
(species key)
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Cow, Pig
(Due to sequence homology)
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a dilution of 1/2000.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Brain, Mouse Brain, Rat Brain
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Cellular localization
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Cytoplasmic
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Research areas
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Immunology >> Immune Interventions >> Transplantation
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Relevance
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The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels including the tetrameric skeletal muscle ryanodine receptor. There is evidence of multiple alternatively spliced transcript variants for this gene, but the full length nature of some variants has not been determined.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.10 - 1.00 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FKBP12 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FKBP12 antibody images, references, products related to ab24373 and other tools.
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FKBP12 antibody images:
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 Western blot - FKBP12 antibody (ab24373)
All lanes : FKBP12 antibody (ab24373) at 1 µg/ml
Lane 1 : Human Brain Tissue Lysate
Lane 2 : Mouse Brain Tissue Lysate
Lane 3 : Rat Brain Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 12 kDa
Observed band size : 12 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FKBP12 antibody (ab24373)
ab24373 staining FKBP12 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed in formaldehyde before heat mediated antigen retrieval and then blocked for 45 minutes at 20°C (5 minutes of peroxidase block and 10 minutes of protein block). The primary antibody was diluted, 1/2000 in PBS and incubated with sample for 45 minutes at 21°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary. This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
See Abreview
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Search PubMed (MEDLINE) for references to FKBP12
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FKBP12 antibody - more information
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Customer reviews (feedback) regarding FKBP12 antibody |
Customer FAQs regarding FKBP12 antibody |
Protocols for FKBP12 antibody |
Price and availability of products related to FKBP12 antibody |
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FKBP12 antibody - related products:
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FKBP12 antibody - other tools:
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Contact Abcam with a Technical Enquiry about FKBP12 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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