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Products:Immunology >> Immune Interventions >> Transplantation
Overview
Product name
Anti-FKBP12 antibody
See all FKBP12 products (6) ...
Description
Rabbit polyclonal to FKBP12
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Cow, Pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FKBP12.
(Peptide available as ab25840.)
Positive control
Human Brain, Mouse Brain, Rat Brain
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/2000
WB: Use a concentration of 1 µg/mlDetects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
Target
Function
May play a role in modulation of ryanodine receptor isoform-1 (RYR-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. There are four molecules of FKBP12 per skeletal muscle RYR. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Sequence similarities
Belongs to the FKBP-type PPIase family. FKBP1 subfamily.
Contains 1 PPIase FKBP-type domain.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP62942
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 12 kDa FK506-binding protein antibody
- 12 kDa FKBP antibody
- FK506 binding protein 1 antibody
see all
Database links
- Entrez Gene: 2280 Human
- Entrez Gene: 14225 Mouse
- Entrez Gene: 25639 Rat
- Omim: 186945 Human
- SwissProt: P18203 Cow
- SwissProt: P62942 Human
- SwissProt: P26883 Mouse
- SwissProt: Q62658 Rat
see all
Anti-FKBP12 antibody images:
Western blot - FKBP12 antibody (ab24373)
All lanes : Anti-FKBP12 antibody (ab24373) at 1 µg/ml
Lane 1 : Human Brain Tissue Lysate
Lane 2 : Mouse Brain Tissue Lysate
Lane 3 : Rat Brain Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 12 kDa
Observed band size : 12 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FKBP12 antibody (ab24373)
ab24373 staining FKBP12 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed in formaldehyde before heat mediated antigen retrieval and then blocked for 45 minutes at 20°C (5 minutes of peroxidase block and 10 minutes of protein block). The primary antibody was diluted, 1/2000 in PBS and incubated with sample for 45 minutes at 21°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
Western blot - Anti-FKBP12 antibody (ab24373)
Anti-FKBP12 antibody (ab24373) at 1 µg/ml + FKBP12 protein (ab56524) at 0.1 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 2 minutes
References for Anti-FKBP12 antibody (ab24373)
ab24373 has not yet been referenced specifically in any publications.
Publishing research using ab24373? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"