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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FKBP12 antibody (ab2918)
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Western blot - FKBP12 antibody (ab2918)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FKBP12 antibody (ab2918)
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Product Name
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FKBP12 antibody
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See all FKBP12 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to FKBP12
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Immunogen
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Synthetic peptide: GVQVETISPGDGR, corresponding to amino acids 1-13 of Human FKBP12.
BLAST 'GVQVETISPGDGR' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat, Dog
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Specificity
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Detects FK506 binding protein 12 kDa (FKBP12).
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (See Abreview)
IHC-Fr: 1/700.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Immunology >> Immune Interventions >> Transplantation
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Relevance
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The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels including the tetrameric skeletal muscle ryanodine receptor. There is evidence of multiple alternatively spliced transcript variants for this gene, but the full length nature of some variants has not been determined.
Immunophilins are a family of soluble receptors capable of binding to one of two major immunosuppressant agents; cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Immunophilins function as cis-trans peptidyl-prolyl isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. Thus, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline-containing proteins.
FKBP12:FK506 complexes inhibit calcineurin, a calcium/calmodulin-dependent serine/threonine phosphatase which blocks T-cell activation by preventing lymphokine gene transcription. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1,4,5-trisphosphate receptor. In interactions with members of the TGF beta family type I receptors, FKBP12 has been shown to exert an inhibitory effect on the signaling pathway.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS, 1 mg/ml BSA, 0.05% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FKBP12 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FKBP12 antibody images, references, products related to ab2918 and other tools.
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FKBP12 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FKBP12 antibody (ab2918)
ab2918 at 1/1000 staining FKBP12 from human penis tissue by IHC-P. The tissue was paraformaldehyde fixed and the slides were incubated in citrate buffer for antigen retrieval and heated for 20 minutes. The tissue was incubated with ab2918 for 24 hours. The secondary antibody used was part of the DAKO ENVISON System. The picture depicts staining of nerve fibers within corporal tissue of the human penis. This image is courtesy of an Abreview submitted by Miss gwen lagoda
See Abreview
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Western blot - FKBP12 antibody (ab2918)
All lanes : FKBP12 antibody (ab2918) at 1/1000 dilution
Lane 1 : Whole tissue lysate, dog left ventricle (heart failure)
Lane 2 : Whole tissue lysate, dog left ventricle (heart failure)
Lane 3 : Whole tissue lysate, dog left ventricle (heart failure)
Lane 4 : Whole tissue lysate, dog left ventricle (heart failure)
Lane 5 : Whole tissue lysate, dog left ventricle (heart failure)
Lane 6 : Whole tissue lysate, dog left ventricle (normal)
Lane 7 : Whole tissue lysate, dog left ventricle (normal)
Lane 8 : Whole tissue lysate, dog left ventricle (normal)
Lane 9 : Whole tissue lysate, dog left ventricle (normal)
Lysates/proteins at 20 µg per lane.
Secondary
HRP conjugated donkey polyclonal antibody
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 12 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutes This image is courtesy of an Abreview submitted by Dr sudhish mishra
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FKBP12 antibody (ab2918)
ab2918 at 1/1000 staining mouse heart tissue sections by IHC-P. The tissue was paraformaldehyde fixed, blocked and then a citrate buffer / heat mediated antigen retireival step was performed. The tissue was incubated with the antibody for 24 hours. An HRP conjugated rabbit polyclonal antibody was used as the secondary. This image is courtesy of an Abreview submitted by Miss gwen lagoda
See Abreview
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Immunocytochemistry/ Immunofluorescence - FKBP12 antibody (ab2918)
ICC/IF image of ab2918 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2918, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for FKBP12 antibody (ab2918)
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This product has been used in: (two most recent references)
De Angelis B et al. Generation of Epstein-Barr-virus-specific cytotoxic T lymphocytes resistant to the immunosuppressive drug tacrolimus (FK506). Blood : (2009). WB; Mouse. PubMed: 19759356
Verheye S et al. Selective clearance of macrophages in atherosclerotic plaques by autophagy. J Am Coll Cardiol 49:706-15 (2007). PubMed: 17291937
See all 3 publication references for this product.
If you publish research using ab2918 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to FKBP12
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FKBP12 antibody - more information
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