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Products:Immunology >> Immune Interventions >> Transplantation
Overview
Product name
Anti-FKBP12 antibody
See all FKBP12 products (6) ...
Description
Rabbit polyclonal to FKBP12
Specificity
Detects FK506 binding protein 12 kDa (FKBP12).
Tested applications
IHC-P, ICC/IF, IP, WB, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Dog, Human
Immunogen
Synthetic peptide: GVQVETISPGDGR, corresponding to amino acids 1-13 of Human FKBP12.
GVQVETISPG DGR
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1 mg/ml BSA, 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
Immunophilins are a family of soluble receptors capable of binding to one of two major immunosuppressant agents; cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Immunophilins function as cis-trans peptidyl-prolyl isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. Thus, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline-containing proteins. FKBP12:FK506 complexes inhibit calcineurin, a calcium/calmodulin-dependent serine/threonine phosphatase which blocks T-cell activation by preventing lymphokine gene transcription. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1,4,5-trisphosphate receptor. In interactions with members of the TGF beta family type I receptors, FKBP12 has been shown to exert an inhibitory effect on the signaling pathway.
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
ICC/IF
IHC-Fr
Application notes
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (See Abreview)
IHC-Fr: 1/700.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May play a role in modulation of ryanodine receptor isoform-1 (RYR-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. There are four molecules of FKBP12 per skeletal muscle RYR. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Sequence similarities
Belongs to the FKBP-type PPIase family. FKBP1 subfamily.
Contains 1 PPIase FKBP-type domain.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP62942
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 12 kDa FK506-binding protein antibody
- 12 kDa FKBP antibody
- FK506 binding protein 1 antibody
see all
Database links
- Entrez Gene: 2280 Human
- Entrez Gene: 14225 Mouse
- Entrez Gene: 25639 Rat
- Omim: 186945 Human
- SwissProt: P62942 Human
- SwissProt: P26883 Mouse
- SwissProt: Q62658 Rat
- Unigene: 471933 Human
see all
Anti-FKBP12 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FKBP12 antibody (ab2918)
ab2918 at 1/1000 staining FKBP12 from human penis tissue by IHC-P. The tissue was paraformaldehyde fixed and the slides were incubated in citrate buffer for antigen retrieval and heated for 20 minutes. The tissue was incubated with ab2918 for 24 hours. The secondary antibody used was part of the DAKO ENVISON System. The picture depicts staining of nerve fibers within corporal tissue of the human penis.
This image is courtesy of an Abreview submitted by Miss gwen lagoda
Western blot - FKBP12 antibody (ab2918)
All lanes : Anti-FKBP12 antibody (ab2918) at 1/1000 dilution
Lane 1 : Whole tissue lysate, dog left ventricle (heart failure)
Lane 2 : Whole tissue lysate, dog left ventricle (heart failure)
Lane 3 : Whole tissue lysate, dog left ventricle (heart failure)
Lane 4 : Whole tissue lysate, dog left ventricle (heart failure)
Lane 5 : Whole tissue lysate, dog left ventricle (heart failure)
Lane 6 : Whole tissue lysate, dog left ventricle (normal)
Lane 7 : Whole tissue lysate, dog left ventricle (normal)
Lane 8 : Whole tissue lysate, dog left ventricle (normal)
Lane 9 : Whole tissue lysate, dog left ventricle (normal)
Lysates/proteins at 20 µg per lane.
Secondary
HRP conjugated donkey polyclonal antibody
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 12 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutes
This image is courtesy of an Abreview submitted by Dr sudhish mishra
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FKBP12 antibody (ab2918)
ab2918 at 1/1000 staining mouse heart tissue sections by IHC-P. The tissue was paraformaldehyde fixed, blocked and then a citrate buffer / heat mediated antigen retireival step was performed. The tissue was incubated with the antibody for 24 hours. An HRP conjugated rabbit polyclonal antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Miss gwen lagoda
Immunocytochemistry/ Immunofluorescence - FKBP12 antibody (ab2918)
ICC/IF image of ab2918 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2918, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-FKBP12 antibody (ab2918)
This product has been referenced in:
- Hwang Yet al. Forced dimerization increases the activity of ?EGFR/EGFRvIII and enhances its oncogenicity. Mol Cancer Res 9:1199-208 (2011). WB; Human.Read more (PubMed: 21775422) »
- Varma Det al. Development and application of in vivo molecular traps reveals that dynein light chain occupancy differentially affects dynein-mediated processes. Proc Natl Acad Sci U S A 107:3493-8 (2010). IP; Rat.Read more (PubMed: 20133681) »
See all 6 publications for this product
Publishing research using ab2918? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"