FKBP12 protein (His tag) (ab61244)

Product Name 

FKBP12 protein (His tag)

Product type 

Proteins and Peptides

Peptide 

Recombinant full length Human FKBP12 (FK506 binding protein 1A, transcript variant 12A) Swiss-Prot ID = P62942 (GenBank Accession No. NM_054014), containing an N-terminal His tag. MW 13.1 kDa. Expressed in Baculovirus infected Sf9 cell expression system.

Tested applications 
(see key)

SDS-PAGE

Application notes 
(see key)

Recommended dilutions
SDS-PAGE: Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Immunology >> Immune Interventions >> Transplantation

Relevance 

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels including the tetrameric skeletal muscle ryanodine receptor. There is evidence of multiple alternatively spliced transcript variants for this gene, but the full length nature of some variants has not been determined.

This protein can be used for testing rapamycin inhibition of mTOR.

Purity 

>90% by SDS-PAGE

Storage buffer 

Preservative: None
Constituents: 50% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM PMSF, 0.1mM EDTA, pH 7.5

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Concentration 

3.500 mg/ml

Storage instructions 

Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

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