Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab24450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use at an assay dependent dilution. See Abreviews.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).Can be blocked with FKBP38 peptide (ab51928).

Target

  • FunctionConstitutively inactive PPiase, which becomes active when bound to calmodulin and calcium. Seems to act as a chaperone for BCL2, targets it to the mitochondria and modulates its phosphorylation state. The BCL2/FKBP8/calmodulin/calcium complex probably interferes with the binding of BCL2 to its targets. The active form of FKBP8 may therefore play a role in the regulation of apoptosis.
  • Tissue specificityWidely expressed. Highest levels seen in the brain.
  • Sequence similaritiesContains 1 PPIase FKBP-type domain.
    Contains 3 TPR repeats.
  • Cellular localizationMitochondrion membrane.
  • Target information above from: UniProt accession Q14318 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • 38 kDa FK 506 binding protein homolog antibody
    • 38 kDa FK506-binding protein antibody
    • 38 kDa FKBP antibody
    • 38kDa antibody
    • FK506 binding protein 8 antibody
    • FK506 binding protein 8, 38kDa antibody
    • FK506 binding protein, 38kDa antibody
    • FK506-binding protein 8 antibody
    • FKBP 38 antibody
    • FKBP-38 antibody
    • FKBP-8 antibody
    • FKBP8 antibody
    • FKBP8_HUMAN antibody
    • FKBPR38 antibody
    • hFKBP38 antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP8 antibody
    • PPIase FKBP8 antibody
    • Rotamase antibody
    • Sam11 antibody
    see all

Anti-FKBP38 antibody images

  • Immunohistochemical analysis of Human penile tissue, staining FKBP38 with ab24450.

    Tissue was fixed with paraformaldehyde and blocked with blocking reagent for 2 hours; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/1000 in diluent) for 18 hours. An undiluted HRP-conjugated anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

References for Anti-FKBP38 antibody (ab24450)

This product has been referenced in:
  • Wiley M  et al. Toxoplasma gondii activates hypoxia-inducible factor (HIF) by stabilizing the HIF-1alpha subunit via type I activin-like receptor kinase receptor signaling. J Biol Chem 285:26852-60 (2010). WB ; Human . Read more (PubMed: 20581113) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (Penile Tissue)
Loading amount 60 µg
Specification Penile Tissue
Gel Running Conditions Reduced Denaturing (15% ge (from another supplier)l)
Blocking step Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. gwen Lagoda

Verified customer

Submitted Jul 28 2008

Application Western blot
Sample Mouse Tissue lysate - whole (Mouse heart and brain)
Loading amount 5 µg
Specification Mouse heart and brain
Gel Running Conditions Reduced Denaturing
Blocking step Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. gwen Lagoda

Verified customer

Submitted Mar 13 2008

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer 1X
Permeabilization No
Blocking step Peroxidase Blocking Reagent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. gwen Lagoda

Verified customer

Submitted Jan 24 2008

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Penile Tissue Sections)
Specification Penile Tissue Sections
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer Diluted 1X
Permeabilization Yes - 0.4% Triton in PBS
Blocking step Peroxidase Blocking Reagent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. gwen Lagoda

Verified customer

Submitted Jan 11 2008

Application Western blot
Sample Rat Tissue lysate - whole (Rat Penile Tissue)
Loading amount 60 µg
Specification Rat Penile Tissue
Gel Running Conditions Reduced Denaturing
Blocking step Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100µg/mL · Temperature: 25°C
Username

Miss. gwen Lagoda

Verified customer

Submitted Jul 27 2007

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Penile Sections)
Specification Penile Sections
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Blocking step Dako Peroxidase Blocking Reagent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100
Username

Miss. gwen Lagoda

Verified customer

Submitted Dec 01 2006

Thank you for your enquiry. We have checked the original film and corrected the molecular weight on the datasheet. The immunogen used was based on the ACCESSION AY225340. Mouse intestine has been used as a positive control. We have not tested an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"