Anti-FKBP38 antibody (ab90530)

Overview

• Product nameAnti-FKBP38 antibodySee all FKBP38 primary antibodies ...
• Description
  Rabbit polyclonal to FKBP38
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat, Cow, Non Human Primates
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human FKBP38.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab99883.)

• Positive controlThis antibody gave a positive signal in Human and Mouse Liver Tissue Lysates.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Trafficking
  • Chaperones
  • Other Chaperones

  • Signal Transduction
  • Signaling Pathway
  • Calcium Signaling
  • Calmodulin Pathway

  • Signal Transduction
  • Signaling Pathway
  • Calcium Signaling
  • Calcium Binding Proteins

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Associated Proteins

Applications

Target

• FunctionConstitutively inactive PPiase, which becomes active when bound to calmodulin and calcium. Seems to act as a chaperone for BCL2, targets it to the mitochondria and modulates its phosphorylation state. The BCL2/FKBP8/calmodulin/calcium complex probably interferes with the binding of BCL2 to its targets. The active form of FKBP8 may therefore play a role in the regulation of apoptosis.
• Tissue specificityWidely expressed. Highest levels seen in the brain.
• Sequence similaritiesContains 1 PPIase FKBP-type domain.
  Contains 3 TPR repeats.
• Cellular localizationMitochondrion membrane.

Target information above from: UniProt accession Q14318 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 23770 Human
  • Entrez Gene: 14232 Mouse
  • Entrez Gene: 290652 Rat
  • Omim: 604840 Human
  • SwissProt: Q14318 Human
  • SwissProt: O35465 Mouse
  • SwissProt: Q3B7U9 Rat
  • Unigene: 173464 Human

  • Unigene: 141864 Mouse
  • Unigene: 99789 Rat

  see all

• Alternative names
  38 kDa FK506-binding protein antibody38 kDa FKBP antibody38kDa antibody

  FK506 binding protein 8 antibodyFK506 binding protein 8, 38kDa antibodyFK506 binding protein, 38kDa antibodyFK506-binding protein 8 antibodyFKBP 38 antibodyFKBP-38 antibodyFKBP-8 antibodyFKBP8 antibodyFKBP8_HUMAN antibodyFKBPR38 antibodyhFKBP38 antibodyPeptidyl-prolyl cis-trans isomerase FKBP8 antibodyPPIase FKBP8 antibodyRotamase antibodySam11 antibody

  see all

Anti-FKBP38 antibody images

• 

  Western blot - FKBP38 antibody (ab90530)
  Anti-FKBP38 antibody (ab90530) at 1 µg/ml + Liver (Human) Tissue Lysate - adult normal tissue (ab29889) at 10 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 38 kDa
  Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 20 minutes
• 

  Western blot - Anti-FKBP38 antibody (ab90530)
  Anti-FKBP38 antibody (ab90530) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 38 kDa
  Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 12 minutes