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Overview

  • Product nameFKBP52 protein (Active)See all FKBP52 proteins and peptides ...
  • Protein descriptionRecombinant full length human FKBP52 fused to His-tag at N-terminus; amino acids 1-459 (479 amino acids), 53.9kDa. Swiss ID = Q02790
  • Expression hostE. coli

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Purification notesab84189 is purified using conventional chromatography techniques. Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method).
  • Biological activitySpecific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH 8.0 using chymotrypsin.

    Activity Assay

    1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
    2. Add 10 µl of recombinant FKBP4 protein with 1 µg in assay buffer.
    3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
    4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
    5. Record the increase in A405 nm for 30 minutes at 25°C.
  • Mass spectrometryMALDI-TOF
  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 10% Glycerol, 20mM Tris, pH 8.0
  • Concentration information loading...
  • Additional notesEndotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method).
  • Sequence notesMGSSHHHHHH SSGLVPRGSH MTAEEMKATE SGAQSAPLPM EGVDISPKQD EGVLKVIKRE GTGTEMPMIG DRVFVHYTGW LLDGTKFDSS LDRKDKFSFD LGKGEVIKAW DIAIATMKVG EVCHITCKPE YAYGSAGSPP KIPPNATLVF EVELFEFKGE DLTEEEDGGI IRRIQTRGEG YAKPNEGAIV EVALEGYYKD KLFDQRELRF EIGEGENLDL PYGLERAIQR MEKGEHSIVY LKPSYAFGSV GKEKFQIPPN AELKYELHLK SFEKAKESWE MNSEEKLEQS TIVKERGTVY FKEGKYKQAL LQYKKIVSWL EYESSFSNEE AQKAQALRLA SHLNLAMCHL KLQAFSAAIE SCNKALELDS NNEKGLFRRG EAHLAVNDFE LARADFQKVL QLYPNNKAAK TQLAVCQQRI RRQLAREKKL YANMFERLAE EENKAKAEAS SGDHPTDTEM KEEQKSNTAG SQSQVETEA
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab84189 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    Functional Studies FuncS: Use at an assay dependent concentration.
    WB WB: Use at an assay dependent concentration. Ab84189 can be used as a WB positive control in conjunction with ab97306.
  • Application notesThis peptide can be used with studies using ab47953,ab81527,ab110191 and ab155937.

    • Protein info

    • Alternative names
        51 kDa FK506-binding protein52 kDa FK506 binding protein52 kDa FK506-binding protein
        52 kDa FKBP59 kDa immunophilinFK506 binding protein 4FK506-binding protein 4FKBP 4FKBP 52FKBP 59FKBP-4FKBP-52FKBP4FKBP4_HUMANFKBP51FKBP52 proteinFKBP59HBIHsp 56HSP binding immunophilinHSP-binding immunophilinHsp56Immunophilin FKBP52N-terminally processedp52p59p59 proteinPeptidyl prolyl cis trans isomerasePeptidyl-prolyl cis-trans isomerase FKBP4Peptidylprolyl cis trans isomerasePPIasePPIase FKBP4RotamaseT cell FK506 binding protein 59kD
      see all
  • FunctionImmunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesContains 2 PPIase FKBP-type domains.
    Contains 3 TPR repeats.
  • DomainThe PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
    The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
    The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400.
  • Post-translational
    modifications    Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton.

    • Target information above from: UniProt accession Q02790 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    FKBP52 protein (Active) images

    • SDS-PAGE - FKBP52 protein (His tag) (ab84189)
      SDS-PAGE - FKBP52 protein (His tag) (ab84189)
      15% SDS-PAGE showing ab84189 at approximately 53.9kDa (3µg).
    • Western blot
      Western blot
      Anti-FKBP52 antibody (ab97306) at 1/3000 dilution + FKBP52 protein (Active) (ab84189) at 0.1 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 30 seconds

    References for FKBP52 protein (Active) (ab84189)

    ab84189 has not yet been referenced specifically in any publications.

    Publishing research using ab84189 ? Please let us know so that we can cite the reference in this datasheet

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