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Overview

  • Product nameFLJ39378 protein (Tagged-His Tag)See all FLJ39378 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 116-403 (288 aa) of Human FLJ39378 with a N terminal His tag; predicted MWt 35kDa: Uniprot ID = Q5EBL4.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitution with 61 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesAQDLLSQIAQLQEENKQLMTNLSHKDVNFSEEEFQKHEGM SERERQVMKKLKEV VDKQRDEIRAKDRELGLKNEDVEA LQQQQTRLMKINHDLRHRVTVVEAQGKALI EQKVELEA DLQTKEQEMGSLRAELGKLRERLQGEHSQNGEEEPETEPV GEESIS DAEKVAMDLKDPNRPRFTLQELRDVLHERNEL KSKVFLLQEELAYYKSEEMEEE NRIPQPPPIAHPRTSP QPESGIKRLFSFFSRDKKRLANTQRNVHIQESFGQWAN THRDDGYTEQGQEALQHL
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91890 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        GOSPELMGC105128MGC99793
        Rab interacting lysosomal protein like 1Rab-interacting lysosomal-like protein 1RILP like protein 1RILP-like protein 1RILPL 1RILPL1RIPL1_HUMANRLP1
      see all
  • FunctionNeuroprotective protein, which acts by sequestring GAPDH in the cytosol and prevent the apoptotic function of GAPDH in the nucleus. Competes with SIAH1 for binding GAPDH (By similarity). Does not regulate lysosomal morphology and distribution.
  • Tissue specificityWidely expressed. Expressed at lower level in liver and kidney.
  • Sequence similaritiesBelongs to the RILPL family.
    Contains 1 RILP-like domain.
  • Post-translational
    modifications    S-nitrosylation is required for the interaction with GAPDH.
  • Cellular localizationCytoplasm > cytosol.

    • Target information above from: UniProt accession Q5EBL4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    FLJ39378 protein (Tagged-His Tag) images

    • SDS-PAGE - FLJ39378 protein (Tagged-His Tag) (ab91890)
      SDS-PAGE - FLJ39378 protein (Tagged-His Tag) (ab91890)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images colored control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for FLJ39378 protein (Tagged-His Tag) (ab91890)

    ab91890 has not yet been referenced specifically in any publications.

    Publishing research using ab91890 ? Please let us know so that we can cite the reference in this datasheet

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