Overview

  • Product nameFLRG Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 250 pg/ml
  • Range
    204.8 pg/ml - 50000 pg/ml
  • Recovery

    89 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.6 67% - 130%
    Serum 95.75 84% - 103%
    Plasma 77.86 67% - 99%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s FLRG Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human in serum, plasma, cell culture supernatants and urine.

    This assay employs an antibody specific for Human FLRG coated on a 96-well plate. Standards and samples are pipetted into the wells and FLRG present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FLRG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FLRG bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab113318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

FLRG Human ELISA Kit images

  • Representative standard curve using ab113318

Protocols

References for FLRG Human ELISA Kit (ab113318)

ab113318 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"