FLRG Human ELISA Kit (ab113318)
- Product nameFLRG Human ELISA Kit
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich (quantitative)
- Sensitivity< 250 pg/ml
- Range204.8 pg/ml - 50000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 94.6 67% - 130% Serum 95.75 84% - 103% Plasma 77.86 67% - 99%
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s FLRG Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human in serum, plasma, cell culture supernatants and urine.
This assay employs an antibody specific for Human FLRG coated on a 96-well plate. Standards and samples are pipetted into the wells and FLRG present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FLRG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FLRG bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human FLRG 2 vials FLRG Microplate (12 x 8 wells) 1 unit Recombinant Human FLRG Standard 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
- Research Areas
- SwissProt: O95633 Human
Our Abpromise guarantee covers the use of ab113318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for FLRG Human ELISA Kit (ab113318)
ab113318 has not yet been referenced specifically in any publications.