Overview

  • Product nameAnti-FNDC3B antibody
    See all FNDC3B primary antibodies
  • Description
    Rabbit polyclonal to FNDC3B
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: SCTTVYNGYG KGHSGGSGGG GSGSGPGIKK TERRARSSPK SNDSDLQEYE , corresponding to internal sequence amino acids 217-266 of Human FNDC3B

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 133 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • RelevanceFNDC3B may be a positive regulator of adipogenesis.
  • Cellular localizationMembrane; Single pass membrane protein
  • Database links
  • Alternative names
    • DKFZp686D14170 antibody
    • DKFZp762K137 antibody
    • Factor for adipocyte differentiation 104 antibody
    • FAD104 antibody
    • fibronectin type III domain containing 3B antibody
    • FLJ23399 antibody
    • HCV NS5A binding protein 37 antibody
    • MGC10002 antibody
    • NS5ABP37 antibody
    • PRO4979 antibody
    • YVTM2421 antibody
    see all

Anti-FNDC3B antibody images

  • Anti-FNDC3B antibody (ab69854) at 2.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 133 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)

References for Anti-FNDC3B antibody (ab69854)

ab69854 has not yet been referenced specifically in any publications.

Product Wall

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