Overview
- Product nameAnti-FNDC3B antibodySee all FNDC3B primary antibodies ...
- DescriptionRabbit polyclonal to FNDC3B
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog - Immunogen
A region within synthetic peptide: SCTTVYNGYG KGHSGGSGGG GSGSGPGIKK TERRARSSPK SNDSDLQEYE , corresponding to internal sequence amino acids 217-266 of Human FNDC3B
- Positive controlJurkat cell lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab69854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | |
| ELISA |
ELISA titre using peptide based assay: 1/312500.
WB: Use at a concentration of 2.5 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 133 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- RelevanceFNDC3B may be a positive regulator of adipogenesis.
- Cellular localizationMembrane; Single pass membrane protein
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Database links
- Entrez Gene: 64778 Human
- Omim: 611909 Human
- SwissProt: Q53EP0 Human
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Alternative names
- DKFZp686D14170 antibodyDKFZp762K137 antibodyFactor for adipocyte differentiation 104 antibody
- FAD104 antibodyfibronectin type III domain containing 3B antibodyFLJ23399 antibodyHCV NS5A binding protein 37 antibodyMGC10002 antibodyNS5ABP37 antibodyPRO4979 antibodyYVTM2421 antibody
see all
Anti-FNDC3B antibody images
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Western blot - FNDC3B antibody (ab69854)Anti-FNDC3B antibody (ab69854) at 2.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk / PBS buffer
Secondary
anti-Rabbit IgG HRP at 1/50000 dilution
Predicted band size : 133 kDa
Observed band size : 44 kDa (why is the actual band size different from the predicted?)
References for Anti-FNDC3B antibody (ab69854)
ab69854 has not yet been referenced specifically in any publications.
