Anti-FOXA2 antibody [1C7] (ab117542)

Overview

• Product nameAnti-FOXA2 antibody [1C7]See all FOXA2 primary antibodies ...
• Description
  Mouse monoclonal [1C7] to FOXA2
• Tested applicationsWB, IP, ELISA, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant Human protein.

  Run BLAST with Run BLAST with
• Positive controlHuman breast tissue. HepG2 cells / cell lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C or -20°C long term. Avoid repeated freeze / thaw cycles. Store undiluted.
• Storage bufferpH: 7.20
  Constituent: 99% PBS
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone number1C7
• IsotypeIgG2a
• Light chain typekappa
• Research Areas
  • Metabolism
  • Types of disease
  • Obesity

  • Developmental Biology
  • Lineage specification
  • Endoderm

  • Stem Cells
  • Lineage Markers
  • Endoderm

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Forkhead Box
  • FOXA

Applications

Target

• FunctionTranscription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
• Sequence similaritiesContains 1 fork-head DNA-binding domain.
• Post-translational
  modificationsPhosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
• Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus.

Target information above from: UniProt accession Q9Y261 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3170 Human
  • Omim: 600288 Human
  • SwissProt: Q9Y261 Human
  • Unigene: 155651 Human

• Alternative names
  Forkhead box A2 antibodyForkhead box protein A2 antibodyFOX A2 antibody

  foxa2 antibodyFOXA2_HUMAN antibodyHepatic nuclear factor 3 beta antibodyHepatocyte nuclear factor 3 antibodyHepatocyte nuclear factor 3 beta antibodyHepatocyte nuclear factor 3-beta antibodyHNF 3B antibodyHNF-3-beta antibodyHNF-3B antibodyHNF3B antibodyMGC19807 antibodyTCF 3B antibodyTCF-3B antibodyTCF3B antibodyTranscription factor 3B antibody

  see all

Anti-FOXA2 antibody [1C7] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [1C7] (ab117542)
  Human Breast, Formalin-Fixed, Paraffin-Embedded tissue, stained with ab117542 at 5µg/ml.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [1C7] (ab117542)
  Immunofluorescence of FOXA2 expression on HepG2 cells with ab117542 at 10 µg/ml.
• 

  Western blot - Anti-FOXA2 antibody [1C7] (ab117542)
  Anti-FOXA2 antibody [1C7] (ab117542) + HepG2 cell lysate at 1/500 dilution
  
  Predicted band size : 48 kDa
  
• 

  ELISA - Anti-FOXA2 antibody [1C7] (ab117542)
  The detection limit forab117542 is approximately 0.03ng/ml as a capture antibody.