Anti-FOXA2 antibody [1C7] (ab117542)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab117542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 48 kDa.
IP Use at an assay dependent dilution. (40 ug of antibody per 100 ul of beads)
ELISA Use at an assay dependent dilution.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionTranscription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
  • Sequence similaritiesContains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Forkhead box A2 antibody
    • Forkhead box protein A2 antibody
    • FOX A2 antibody
    • foxa2 antibody
    • FOXA2_HUMAN antibody
    • Hepatic nuclear factor 3 beta antibody
    • Hepatocyte nuclear factor 3 antibody
    • Hepatocyte nuclear factor 3 beta antibody
    • Hepatocyte nuclear factor 3-beta antibody
    • HNF 3B antibody
    • HNF-3-beta antibody
    • HNF-3B antibody
    • HNF3B antibody
    • MGC19807 antibody
    • TCF 3B antibody
    • TCF-3B antibody
    • TCF3B antibody
    • Transcription factor 3B antibody
    see all

Anti-FOXA2 antibody [1C7] images

  • Human Breast, Formalin-Fixed, Paraffin-Embedded tissue, stained with ab117542 at 5µg/ml.
  • Immunofluorescence of FOXA2 expression on HepG2 cells with ab117542 at 10 µg/ml.
  • Anti-FOXA2 antibody [1C7] (ab117542) + HepG2 cell lysate at 1/500 dilution

    Predicted band size : 48 kDa
  • The detection limit forab117542 is approximately 0.03ng/ml as a capture antibody.

References for Anti-FOXA2 antibody [1C7] (ab117542)

ab117542 has not yet been referenced specifically in any publications.

Product Wall

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I have created a FOCR order with order number 1189454. The new lot will be available for shipping on 6th November. Thank you very much for having patience.

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The product as we agreed ab117542 is out of stock since last week, which is why the shipment was delayed. We are still waiting for this product to be stock however it can take 1-2 weeks more. We apologize for delay...

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I think there was a order mix up, because the order number MLZ969044 which I used to send you the ab117542 have only one ab89997 which means second cannot be issue.
The order MLZ945441 have 2 vials of ab89997. C...

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Wie versprochen habe ich alle unsere FOXA2-Antikörper darauf geprüft, welche Isoformen von FOXA2 sie erkennen. Leider sind alle Immunogene in Regionen lokalisiert, die in beiden Isoformen vorkommen (Details siehe...

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