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Product name
Anti-FOXA2 antibody
See all FOXA2 products (12) ...
Description
Rabbit polyclonal to FOXA2
Specificity
The winged helix transcription factor, FOXA2, is expressed in the foregut endoderm and in subsets of respiratory epithelial cells in the fetal and adult lung. It should be known that FOXA2 is not normally detected in wildtype tissue in western blot. A positive control for western blot would be a cell line that is over-expressing FOXA2, ie: transfected to over-express FOXA2.
Tested applications
IHC-Fr, WB, IHC-P, IHC-FoFr, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Recombinant Fusion protein: MEGHEPSDWS SYYAEPEGYS SVSNMNASLG MNGMNTYMSM SAAAMGSGSG NMSAGSMNMS SYVGAGMSPS LAGMSPGAGA , corresponding to N terminal amino acids 7-86 of Rat FOXA2
MEGHEPSDWSSYYAEPEGYSSVSNMNASLGMNGMNTYMSM SAAAMGSGSGNMSAGSMNMSSYVGAGMSPSLAGMSPGAGA
Positive control
IHC: Adult mouse lung tissue. WB: transfected cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Obesity
Developmental Biology >> Lineage specification >> Endoderm
Stem Cells >> Lineage Markers >> Endoderm
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box >> FOXA
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/1000. Immerse sections in 10 mM sodium citrate, pH 6.0, and heat in a microwave for 15 min at 90%deg;C.Block sections in 2-4% normal serum in PBS + 0.2% Triton X-100 for 2 hr at room temperature.Incubate primary antibody in blocking buffer.
WB: 1/5000. Predicted molecular weight: 48 kDa.Block in 10% milk in TBST buffer (10 mM Tris-HCl, pH 7.4, 0.9% NaCl, 0.2% Tween20) 3 times 20 minutes.
IHC-P: 1/2000 - 1/4000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-FoFr: Use at an assay dependent dilution.
ICC/IF: 1/1000.
Target
Function
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
Sequence similarities
Contains 1 fork-head DNA-binding domain.
Post-translational
modifications
Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus.
Target information above from: UniProt accessionQ9Y261
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Forkhead box A2 antibody
- Forkhead box protein A2 antibody
- FOX A2 antibody
see all
Database links
- Entrez Gene: 3170 Human
- Entrez Gene: 15376 Mouse
- Entrez Gene: 25099 Rat
- Omim: 600288 Human
- SwissProt: Q9Y261 Human
- SwissProt: P35583 Mouse
- SwissProt: P32182 Rat
- Unigene: 155651 Human
see all
Anti-FOXA2 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FOXA2 antibody (ab40874)
Immunohistochemistry. Adult mouse lung was embedded in paraffin and stained with ab40874 (1:2000 dilution). FOXA2 was visualized by DAB (black color) and counterstained with Nuclear Fast Red (pink color). Magnification 20x.
Immunohistochemistry (Frozen sections) - FOXA2 antibody (ab40874)
ab40874 at a 1/1000 dilution staining FOXA2 in rat midbrain tissue sections by Immunohistochemistry (frozen sections) incubated for 16 hours at 25°C. PFA fixed. Blocked with 5% serum for 1 hour at 25°C. Secondary used at 1/200 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 488. DAPI staining (blue), FOXA2 staining (green).
This image is courtesy of an anonymous abreview.
Immunohistochemistry (PFA perfusion fixed frozen sections) - FOXA2 antibody (ab40874)
ab40874 staining FOXA2 in mouse embryo by Immunohistochemistry (PFA perfusion fixed frozen sections). Embryos were isolated, fixed for 20 minutes in 2% PFA in PBS, and then permeabilized in 0.1% Triton X-100 in 0.1 M glycine pH 8.0. After blocking in 10% FCS, 3% goat serum, 0.1% BSA, 0.1% Tween 20 for 2 hours, embryos were incubated with the primary antibody overnight at 4°C in blocking solution. After several washes in PBS containing 0.1% Tween-20 (PBST) samples were incubated with Donkey anti-rabbit 488 secondary antibodies.
A. E7.0 (Mid streak embryos) B. E7.0 (Late streak embryos)
Image from Burtscher Ingo et.al., Development. 2009 Mar;136(6):1029-38 Fig 1.
Immunocytochemistry/ Immunofluorescence - FOXA2 antibody (ab40874)
ICC/IF image of ab40874 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40874, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-FOXA2 antibody (ab40874)
This product has been referenced in:
- Viotti M et al. Role of the gut endoderm in relaying left-right patterning in mice. PLoS Biol 10:e1001276 (2012). ICC/IF; Mouse.Read more (PubMed: 22412348) »
- Pulina MV et al. Essential roles of fibronectin in the development of the left-right embryonic body plan. Dev Biol 354:208-20 (2011). ICC/IF; Mouse.Read more (PubMed: 21466802) »
See all 12 publications for this product
Publishing research using ab40874? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"