Overview
- Product nameAnti-FOXA2 antibodySee all FOXA2 primary antibodies ...
- DescriptionGoat polyclonal to FOXA2
- SpecificityThis antibody is expected to recognise both reported isoforms.
- Tested applicationsICC/IF, WB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Xenopus laevis - Immunogen
Synthetic peptide: GVYSRPIMNSS, corresponding to amino acids 447-457 of Human FOXA 2.
- Positive controlHuman liver lysate
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab5074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: 1/50. PubMed: 19564924 |
| WB | WB: Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).Can be blocked with FOXA2 peptide (447-457) (ab23065). A nuclear extraction procedure may be required to concentrate the protein in samples. |
Target
- FunctionTranscription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
- Sequence similaritiesContains 1 fork-head DNA-binding domain.
- Post-translational
modificationsPhosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation. - Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus.
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Database links
- Entrez Gene: 3170 Human
- Entrez Gene: 15376 Mouse
- Entrez Gene: 25099 Rat
- Omim: 600288 Human
- SwissProt: Q9Y261 Human
- SwissProt: P35583 Mouse
- SwissProt: P32182 Rat
- Unigene: 155651 Human
- Unigene: 938 Mouse
- Unigene: 10948 Rat
see all
Target information above from: UniProt accession
Q9Y261
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Forkhead box A2 antibodyForkhead box protein A2 antibodyFOX A2 antibody
- FOXA2 antibodyFOXA2_HUMAN antibodyHepatic nuclear factor 3 beta antibodyHepatocyte nuclear factor 3 antibodyHepatocyte nuclear factor 3 beta antibodyHepatocyte nuclear factor 3-beta antibodyHNF 3B antibodyHNF-3-beta antibodyHNF-3B antibodyHNF3B antibodyMGC19807 antibodyTCF 3B antibodyTCF-3B antibodyTCF3B antibodyTranscription factor 3B antibody
see all
Anti-FOXA2 antibody images
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Western blot - FOXA2 antibody (ab5074)Anti-FOXA2 antibody (ab5074) at 1 µg/ml + human liver lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 48 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Primary incubated for 1 hour.
References for Anti-FOXA2 antibody (ab5074)
This product has been referenced in:
- Rashid ST et al. Modeling inherited metabolic disorders of the liver using human induced pluripotent stem cells. J Clin Invest 120:3127-36 (2010). ICC/IF ; Human . Read more (PubMed: 20739751) »
- Vallier L et al. Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways. PLoS One 4:e6082 (2009). ICC/IF ; Human . Read more (PubMed: 19564924) »
