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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Overview
Product name
Anti-FOXC2 antibody - ChIP Grade
See all FOXC2 products (4) ...
Description
Goat polyclonal to FOXC2 - ChIP Grade
Specificity
The antibody labels FOXC2 in mouse lymphatic primordia, jugular lymph sacs, lymphatic collectors and capillaries. In western blotting the antibody has been tested on FOXC2 overexpressing cell lines but not on endogenous protein.
Tested applications
WB, ChIP, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: RHAAPYSYDCTKY, corresponding to C terminal amino acids 489-501 of Human FOXC2.
RHAAPYSYDC TKY
Positive control
IHC: mouse lymphatic primordia, jugular lymph sacs, lymphatic collectors and capillaries WB: FOXC2 overexpressing cell lines
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Excretory system development >> Kidney development
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box >> FOXC
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP
Application notes
ChIP: Use at an assay dependent dilution (PMID 18579532).
IHC-P: 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa).
May be unsuitable for IHC-Fr according to a customer Abreview.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcriptional activator. Might be involved in the formation of special mesenchymal tissues.
Involvement in disease
Defects in FOXC2 are the cause of lymphedema hereditary type 2 (LMPH2) [MIM:153200]; also known as Meige lymphedema. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections, and physical impairment.
Defects in FOXC2 are a cause of lymphedema-yellow nails (LYYN) [MIM:153300]. LYYN is characterized by yellow, dystrophic, thick and slowly growing nails, associated with lymphedema and respiratory involvement. Lymphedema occurs more often in the lower limbs. It can appear at birth or later in life. Onset generally follows the onset of ungual abnormalities.
Defects in FOXC2 are a cause of lymphedema-distichiasis (LYD) [MIM:153400]. LYD is characterized by primary limb lymphedema usually starting at puberty (but in some cases later or at birth) and associated with distichiasis (double rows of eyelashes, with extra eyelashes growing from the Meibomian gland orifices).
Sequence similarities
Contains 1 fork-head DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ99958
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Drosphilia Forkhead Homolog Like 14 antibody
- FKHL 14 antibody
- FKHL14 antibody
see all
Database links
- Entrez Gene: 2303 Human
- Entrez Gene: 14234 Mouse
- GenBank: NM_005251 Human
- GenBank: NM_013519 Mouse
- Omim: 602402 Human
- SwissProt: Q99958 Human
- SwissProt: Q61850 Mouse
- Unigene: 239571 Human
see all
Anti-FOXC2 antibody - ChIP Grade images:
Western blot - FOXC2 antibody (ab5060)
Predicted band size : 56 kDa
FoxC2 is highly expressed in COS7 and 293 cells transiently transfected with pcDNA-FOXC2. This figure shows a western blot incubated with the ab5060 antibody after running lysates from COS7 and 293 cells transiently expressing FoxC2 (lane 1 and 3 respectively). Lane 2 shows a negative control of 293 cells transfected with an empty vector. Review by Michal Bell submitted 19 August 2004
Western blot - FOXC2 antibody - ChIP Grade (ab5060)
Anti-FOXC2 antibody - ChIP Grade (ab5060) at 1 µg/ml + Human breast cancer lysates at 30 µg
developed using the ECL technique
Predicted band size : 56 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
The primary antibody incubated for 1 hour.
References for Anti-FOXC2 antibody - ChIP Grade (ab5060)
This product has been referenced in:
- Acharya Met al. A complex regulatory network of transcription factors critical for ocular development and disease. Hum Mol Genet 20:1610-24 (2011).Read more (PubMed: 21282189) »
- Nakao Set al. Discontinuous LYVE-1 expression in corneal limbal lymphatics: dual function as microvalves and immunological hot spots. FASEB J : (2011).Read more (PubMed: 22090317) »
See all 9 publications for this product
Publishing research using ab5060? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"