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Western blot - FOXC2 antibody (ab5060)
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Western blot - FOXC2 antibody - ChIP Grade (ab5060)
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Product Name
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FOXC2 antibody - ChIP Grade
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See all FOXC2 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to FOXC2 - ChIP Grade
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Immunogen
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Synthetic peptide: RHAAPYSYDCTKY, corresponding to C terminal amino acids 489-501 of Human FOXC2.
BLAST 'RHAAPYSYDCTKY' with  or 
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Reacts with
(species key)
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Hu, Ms
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Specificity
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The antibody labels FOXC2 in mouse lymphatic primordia, jugular lymph sacs, lymphatic collectors and capillaries. In western blotting the antibody has been tested on FOXC2 overexpressing cell lines but not on endogenous protein.
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Tested applications
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ChIP, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use at an assay dependent dilution (PMID 18579532).
IHC-P: 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa).
May be unsuitable for IHC-Fr according to a customer Abreview.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
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IHC: mouse lymphatic primordia, jugular lymph sacs, lymphatic collectors and capillaries
WB: FOXC2 overexpressing cell lines
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Organogenesis >> Excretory system development >> Kidney development
Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box >> FOXC
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Relevance
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FOXC2 belongs to the forkhead family of transcription factors which is characterized by a distinct DNA binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FOXC2 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FOXC2 antibody - ChIP Grade images, references, products related to ab5060 and other tools.
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FOXC2 antibody - ChIP Grade images:
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 Western blot - FOXC2 antibody (ab5060)
Predicted band size : 56 kDa
FoxC2 is highly expressed in COS7 and 293 cells transiently transfected with pcDNA-FOXC2. This figure shows a western blot incubated with the ab5060 antibody after running lysates from COS7 and 293 cells transiently expressing FoxC2 (lane 1 and 3 respectively). Lane 2 shows a negative control of 293 cells transfected with an empty vector. Review by Michal Bell submitted 19 August 2004
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Western blot - FOXC2 antibody - ChIP Grade (ab5060)
FOXC2 antibody - ChIP Grade (ab5060) at 1 µg/ml + Human breast cancer lysates at 30 µg
developed using the ECL technique
Predicted band size : 56 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
The primary antibody incubated for 1 hour.
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References for FOXC2 antibody - ChIP Grade (ab5060)
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This product has been used in: (two most recent references)
Hayashi H & Kume T Foxc transcription factors directly regulate Dll4 and Hey2 expression by interacting with the VEGF-Notch signaling pathways in endothelial cells. PLoS ONE 3:e2401 (2008). ChIP; Mouse. PubMed: 18545664
Hayashi H et al. The Foxc2 transcription factor regulates angiogenesis via induction of integrin beta3 expression. J Biol Chem 283:23791-800 (2008). ChIP; Mouse. PubMed: 18579532
See all 4 publication references for this product.
If you publish research using ab5060 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to FOXC2
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FOXC2 antibody - ChIP Grade - more information
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Customer reviews (feedback) regarding FOXC2 antibody - ChIP Grade |
Customer FAQs regarding FOXC2 antibody - ChIP Grade |
Protocols for FOXC2 antibody - ChIP Grade |
Price and availability of products related to FOXC2 antibody - ChIP Grade |
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FOXC2 antibody - ChIP Grade - related products:
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FOXC2 antibody - ChIP Grade - other tools:
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Contact Abcam with a Technical Enquiry about FOXC2 antibody - ChIP Grade |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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