Overview
- Product nameAnti-FOXF1 antibodySee all FOXF1 primary antibodies ...
- DescriptionRabbit polyclonal to FOXF1
- Tested applicationsICC/IF, IHC-P, WB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Chicken, Cow, Dog, Xenopus laevis - Immunogen
Synthetic peptide: SASAALNSGA SYIKQQPLSP CNPAANPLSG SLSTHSLEQP YLHQNSHNAP AELQG, corresponding to amino acids 273-327 of FOXF1
- Positive controlFetal placental lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab23194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use at an assay dependent concentration. PubMed: 21341990 |
| IHC-P | IHC-P: Use at an assay dependent dilution. PubMed: 20722018 |
| WB | WB: Use a concentration of 2.5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
Target
- FunctionProbable transcription activator for a number of lung-specific genes.
- Tissue specificityLung and placenta.
- Involvement in diseaseDefects in FOXF1 are the cause of alveolar capillary dysplasia with misalignment of pulmonary veins (ACDMPV) [MIM:265380]. ACDMPV is a rare malformation due to abnormal development of the capillary vascular system in the lungs. Histologically, it is characterized by failure of formation and ingrowth of alveolar capillaries, medial muscular thickening of small pulmonary arterioles with muscularization of the intraacinar arterioles, thickened alveolar walls, and anomalously situated pulmonary veins running alongside pulmonary arterioles and sharing the same adventitial sheath. Less common features include a reduced number of alveoli and a patchy distribution of the histopathologic changes. Affected infants present with respiratory distress and the disease is fatal within the newborn period. Additional features include multiple congenital anomalies affecting the cardiovascular, gastrointestinal, genitourinary, and musculoskeletal systems, as well as disruption of the normal right-left asymmetry of intrathoracic or intraabdominal organs. ACDMPV is a rare cause of persistent pulmonary hypertension of the newborn, an abnormal physiologic state caused by failure of transition of the pulmonary circulation from the high pulmonary vascular resistance of the fetus to the low pulmonary vascular resistance of the newborn.
- Sequence similaritiesContains 1 fork-head DNA-binding domain.
- DomainActivation domains C-terminal of (and distinct from) the forkhead domains are necessary for transcriptional activation.
- Cellular localizationNucleus.
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Database links
- Entrez Gene: 395624 Chicken
- Entrez Gene: 2294 Human
- Entrez Gene: 15227 Mouse
- Entrez Gene: 399398 Xenopus laevis
- Omim: 601089 Human
- SwissProt: Q12946 Human
- SwissProt: Q61080 Mouse
- Unigene: 155591 Human
- Unigene: 33897 Mouse
- Unigene: 390071 Mouse
see all
Target information above from: UniProt accession
Q12946
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- AI450827 antibodyFKH L5 antibodyFKHL 5 antibody
- FKHL5 antibodyFKHL5 antibodyForkhead (Drosophila) like 5 antibodyforkhead (Drosophila)-like 5 antibodyforkhead (Drosophila)-like 5 antibodyforkhead box F1 antibodyforkhead box F1 antibodyForkhead box protein F1 antibodyForkhead drosophila homolog like 5 antibodyForkhead like 5 antibodyForkhead related activator 1 antibodyForkhead related protein FKHL5 antibodyForkhead related transcription factor 1 antibodyforkhead-related activator 1 antibodyforkhead-related activator 1 antibodyForkhead-related protein FKHL5 antibodyForkhead-related transcription factor 1 antibodyFOX F1 antibodyFOXF 1 antibodyfoxf1 antibodyFOXF1_HUMAN antibodyFREAC 1 antibodyFREAC-1 antibodyFREAC1 antibodyFREAC1 antibodyHFH 8 antibodyhomolog-like 5 antibodyhomolog-like 5 antibodyMGC105125 antibody
see all
Anti-FOXF1 antibody images
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Western blot - FOXF1 antibody (ab23194)Anti-FOXF1 antibody (ab23194) at 1.2 µg/ml + Placenta
Predicted band size : 40 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
References for Anti-FOXF1 antibody (ab23194)
This product has been referenced in:
- Bozyk PD et al. Mesenchymal stromal cells from neonatal tracheal aspirates demonstrate a pattern of lung-specific gene expression. Stem Cells Dev 20:1995-2007 (2011). ICC/IF ; Human . Read more (PubMed: 21341990) »
- Minogue BM et al. Characterization of the human nucleus pulposus cell phenotype and evaluation of novel marker gene expression to define adult stem cell differentiation. Arthritis Rheum 62:3695-705 (2010). IHC-P ; Human . Read more (PubMed: 20722018) »
