Overview

  • Product nameAnti-FOXJ2 antibodySee all FOXJ2 primary antibodies ...
  • Description
    Rabbit polyclonal to FOXJ2
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    A region within synthetic peptide: MASDLESSLT SIDWLPQLTL RATIEKLGSA SQAGPPGSSR KCSPGSPTDP, corresponding to N terminal amino acids 1-50 of Human FOXJ2

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

Target

Anti-FOXJ2 antibody images

  • Lane 1 : MW ladder
    Lane 2 : Anti-FOXJ2 antibody (ab49160) at 0.25 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution


    Predicted band size : 62 kDa
    Observed band size : 62 kDa

References for Anti-FOXJ2 antibody (ab49160)

ab49160 has not yet been referenced specifically in any publications.

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