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Anti-FOXO1 + FOXO3 + FOXO4 antibody (ab58518)

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Overview

Product name

Anti-FOXO1 + FOXO3 + FOXO4 antibody
See all FOXO1 + FOXO3 + FOXO4 products (2) ...

Description

Rabbit polyclonal to FOXO1 + FOXO3 + FOXO4

Tested applications

IHC-P, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthetic non phosphopeptide derived from human FOXO1/3/4 around the phosphorylation site of threonine 24/32 (S-C-TP-W-P).

Positive control

IHC-P: Human breast carcinoma tissue.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Clonality

Polyclonal

Isotype

IgG

  • Immunohistochemistry (Paraffin-embedded sections) - FOXO1 + FOX3 + FOX4 antibody (ab58518)Immunohistochemistry (Paraffin-embedded sections) - FOXO1 + FOX3 + FOX4 antibody (ab58518) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab58518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

  • ELISA

     ELISA: 1/5000

    ELISA: 1/5000

Target

Relevance

FOXO1 + FOX3 + FOX4 belong to the forkhead family of transcription factors. FOXO1 (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined. FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia. FOX4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.

Cellular localization

FOXO1: Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus. FOX3: Cytoplasm, cytosol. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. FOX4: Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation.

Alternative names

  • AFX1 antibody
  • FKH1 antibody
  • FKHRL1 antibody
  • FKHRL1P2 antibody
  • Forkhead box O1 antibody
  • Forkhead box O3 antibody
  • Forkhead box O4 antibody
  • FOXO1 antibody
  • FOXO2 antibody
  • FOXO3 antibody
  • FOXO4 antibody
  • MLLT7 antibody
see all

Anti-FOXO1 + FOXO3 + FOXO4 antibody images:

  Immunohistochemistry (Paraffin-embedded sections) - FOXO1 + FOX3 + FOX4 antibody (ab58518)

Immunohistochemistry (Paraffin-embedded sections) - FOXO1 + FOX3 + FOX4 antibody (ab58518)

This image shows human breast carcinoma tissue stained with ab58518 at 1/50 dilution. Right hand image; tissue treated with immunising non phosphopeptide. Left hand image; without immunising non phosphopeptide.

References for Anti-FOXO1 + FOXO3 + FOXO4 antibody (ab58518)

ab58518 has not yet been referenced specifically in any publications.

Publishing research using ab58518? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"