FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) antibody (ab58517)

Product Name 

FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32)

Immunogen 

Synthesized phosphopeptide derived from human FOXO1/3/4 around the phosphorylation site of threonine 24/32 (S-C-T^P-W-P).

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab58517 detects endogenous levels of FOXO1/3/4 only when phosphorylated at threonine 24/32.

Tested applications 
(see key)

ELISA, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/5000.
IHC-P: 1/50 - 1/100.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

IHC-P: human breast carcinoma tissue.

Cellular localization 

FOXO1: Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
FOX3: Cytoplasm, cytosol. Translocates to the nucleus upon oxidative stress and in the absence of survival factors.
FOX4: Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation.

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Chromatin and Nuclear Signaling >> Cardiovascular/Immune >> Hypoxia >> Hypoxia regulated
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box >> FOXO
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins

Relevance 

FOXO1 + FOX3 + FOX4 belong to the forkhead family of transcription factors.
FOXO1 (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined.
FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia.
FOX4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against the non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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