Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) antibody
See all FOXO1 + FOXO3 + FOXO4 products (2) ...
Description
Rabbit polyclonal to FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32)
Specificity
ab58517 detects endogenous levels of FOXO1/3/4 only when phosphorylated at threonine 24/32.
Tested applications
IHC-P, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthesized phosphopeptide derived from human FOXO1/3/4 around the phosphorylation site of threonine 24/32 (S-C-TP-W-P).
Positive control
IHC-P: human breast carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against the non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Epigenetics and Nuclear Signaling >> Cardiovascular/Immune >> Hypoxia >> Hypoxia regulated
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box >> FOXO
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/5000ELISA: 1/5000
WB: 1/500 - 1/1000.
Target
Relevance
FOXO1 + FOX3 + FOX4 belong to the forkhead family of transcription factors. FOXO1 (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined. FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia. FOX4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.
Cellular localization
FOXO1: Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus. FOX3: Cytoplasm, cytosol. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. FOX4: Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation.
Alternative names
- AFX1 antibody
- FKH1 antibody
- FKHRL1 antibody
see all
Database links
- Entrez Gene: 2308 Human
- Entrez Gene: 2309 Human
- Entrez Gene: 4303 Human
- Entrez Gene: 54601 Mouse
- Entrez Gene: 56458 Mouse
- Entrez Gene: 56484 Mouse
- Entrez Gene: 294515 Rat
- Entrez Gene: 302415 Rat
see all
Anti-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - FOXO1 + FOX3 + FOX4 (phospho T24 + T32) antibody (ab58517)
This image shows human breast carcinoma tissue stained with ab58517 at a 1/50 dilution. Right hand image; with immunising phosphopeptide. Left hand image; without immunising phosphopeptide.
Western blot - FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) antibody (ab58517)
All lanes : Anti-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) antibody (ab58517)
Lane 1 : 293 cells
Lane 2 : 293 cells with blocking phosphopeptide
Western blot analysis of extracts from 293 cells, treated with Serum (20%, 15mins), using FOXO1/3/4-pan (Phospho-Thr24/32) antibody.
References for Anti-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) antibody (ab58517)
ab58517 has not yet been referenced specifically in any publications.
Publishing research using ab58517? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"