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Product name
Anti-FOXO1A antibody - ChIP Grade
See all FOXO1A products (21) ...
Description
Rabbit polyclonal to FOXO1A - ChIP Grade
Specificity
ab39670 detects endogenous levels of total FOXO1A.
Tested applications
ICC/IF, WB, IHC-P, ELISA, ChIP, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic non-phosphopeptide derived from human FOXO1A around the phosphorylation site of serine 319.
Positive control
HeLa and 293 cell extracts and human breast carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Obesity
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Coactivators and repressors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box >> FOXO
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Co-activators/co-repressors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 - 5 µg/ml.
WB: 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/20000.ELISA: 1/20000.
ChIP: Use at an assay dependent dilution. PubMed: 20467422See Abreview.
IHC-P: Use at an assay dependent concentration.
Target
Function
Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
Tissue specificity
Ubiquitous.
Involvement in disease
Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
Sequence similarities
Contains 1 fork-head DNA-binding domain.
Post-translational
modifications
Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
Target information above from: UniProt accessionQ12778
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FKH 1 antibody
- FKH1 antibody
- FKH1 antibody
see all
Database links
- Entrez Gene: 2308 Human
- Entrez Gene: 56458 Mouse
- Entrez Gene: 84482 Rat
- Omim: 136533 Human
- SwissProt: Q12778 Human
- SwissProt: Q9R1E0 Mouse
- Unigene: 370666 Human
- Unigene: 29891 Mouse
see all
Anti-FOXO1A antibody - ChIP Grade images:
Immunohistochemistry (Paraffin-embedded sections) - FOXO1A antibody (ab39670)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab39670. (Left image: without peptide; Right image: with peptide 1µg/ml).
Western blot - FOXO1A antibody (ab39670)
Lanes 1 - 3 : Anti-FOXO1A antibody - ChIP Grade (ab39670) at 1/500 dilution
Lanes 4 - 5 : Anti-FOXO1A (phospho S319) antibody (ab38516) at 1/500 dilution
Lane 1 : 293 Cell extract
Lane 2 : HeLa Cell extract
Lane 3 : HeLa Cell extract
Lane 4 : HeLa Cell extract
Lane 5 : HeLa Cell extract
Lysates/proteins at 30 µg per lane.
Secondary
Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)
Predicted band size : 70 kDa
Observed band size : 70 kDa
Immunocytochemistry/ Immunofluorescence-FOXO1A antibody(ab39670)
ICC/IF image of ab39670 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39670, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - FOXO1A antibody (ab39670)
Anti-FOXO1A antibody - ChIP Grade (ab39670) at 1/1000 dilution + Whole cell lysates prepared from human Jurkat cells at 200000 cells
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/2000 dilution
developed using the ECL technique
Predicted band size : 70 kDa
Observed band size : 70 kDa
Additional bands at : 45 kDa (possible non-specific binding),50 kDa (possible non-specific binding),80 kDa (possible non-specific binding).
Exposure time : 30 seconds
Primary diluted in PBS (BSA + 0.1% Tween20) and incubated with sample for 90 minutes at 20°C.
The image is a courtesy of an anonymous abreview.
References for Anti-FOXO1A antibody - ChIP Grade (ab39670)
This product has been referenced in:
- Lin KK et al. CD81 is essential for the re-entry of hematopoietic stem cells to quiescence following stress-induced proliferation via deactivation of the Akt pathway. PLoS Biol 9:e1001148 (2011). IHC-P; Human.Read more (PubMed: 21931533) »
- Wang L et al. Intersection of FOXO- and RUNX1-mediated gene expression programs in single breast epithelial cells during morphogenesis and tumor progression. Proc Natl Acad Sci U S A 108:E803-12 (2011). ChIP; Human.Read more (PubMed: 21873240) »
See all 5 publications for this product
Publishing research using ab39670? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"