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Product name
Anti-FOXO3A antibody
See all FOXO3A products (19) ...
Description
Rabbit polyclonal to FOXO3A
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Dog
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human FOXO3A.
(Peptide available as ab240 31.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: Jurkat (Human T cell lymphoblast-like cell line) This antibody gave a positive signal in the following tissue lysates: Mouse Heart Mouse Skeletal Muscle Rat Heart
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box >> FOXO
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 71 kDa).Can be blocked with FOXO3A peptide (ab24031).
ICC/IF: Use a concentration of 5 µg/ml.
Target
Function
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'.
Tissue specificity
Ubiquitous.
Involvement in disease
Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX.
Sequence similarities
Contains 1 fork-head DNA-binding domain.
Post-translational
modifications
In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1.
Cellular localization
Cytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors.
Target information above from: UniProt accessionO43524
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AF6q21 antibody
- AF6q21 antibody
- AF6q21 protein antibody
see all
Database links
- Entrez Gene: 481954 Dog
- Entrez Gene: 2309 Human
- Entrez Gene: 56484 Mouse
- Entrez Gene: 294515 Rat
- Omim: 602681 Human
- SwissProt: O43524 Human
- SwissProt: Q9WVH4 Mouse
- SwissProt: D3ZBQ1 Rat
see all
Anti-FOXO3A antibody images:
Western blot - FOXO3A antibody (ab23683)
All lanes : Anti-FOXO3A antibody (ab23683) at 1 µg/ml
Lane 1 :
Lane 2 :
Lysates/proteins at 20 µg per lane.
Predicted band size : 71 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa (possible cleavage fragment,cross reactivity).
ab23683 detects a band at approximately 90 kDa that corresponds in size to that seen for FOXO3A. This protein has been shown to migrate at a size larger than the predicted molecular weight (see Yin et al., J. Biol. Chem., Vol. 279, Issue 44, 45721-45727). It also detects a band at 50 kDa which could be a cleavage fragment. Both bands are partially blocked by the addition of the immunizing peptide.
Western blot - FOXO3A antibody (ab23683)
All lanes : Anti-FOXO3A antibody (ab23683) at 1 µg/ml ab23683 detects a band at approximately 90 kDa that corresponds in size to that seen for FOXO3A. This protein has been shown to migrate at a size larger than the predicted molecular weight (see Yin et al., J. Biol. Chem., Vol. 279, Issue 44, 45721-45727).
Lane 1 : Heart (Mouse) Tissue Lysate - normal tissue
Lane 2 :
Lane 3 : Heart (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size : 71 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 16 kDa,27 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - FOXO3A antibody (ab23683)
ICC/IF image of ab23683 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23683, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% HepG2 fixed (10 min) HepG2 cells at 5µg/ml.
References for Anti-FOXO3A antibody (ab23683)
ab23683 has not yet been referenced specifically in any publications.
Publishing research using ab23683? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"