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Anti-FOXO4 antibody (ab12075)

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    Overview

    Product name

    Anti-FOXO4 antibody
    See all FOXO4 products (16) ...

    Description

    Rabbit polyclonal to FOXO4

    Tested applications

    WB, IFmore details

    Cross reactivity

    Reacts with

    Human

    Immunogen

    Synthetic peptide, corresponding to C-term amino acids 451-466 with N-terminally added lysine, of Human AFX / FOXO4.

    Positive control

    AFX transfected COS-7 cells.

    General notes

    Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

    Properties

    Form

    Liquid

    Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Storage buffer

    Preservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.01M PBS, pH 7.4

    Concentration

    Concentration information loading...

    Purity

    Immunogen affinity purified

    Purification notes

    Ab12075 is affinity-purified using the immunizing peptide immobilized on agarose.

    Clonality

    Polyclonal

    Isotype

    IgG

    Research areas

    Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box >> FOXO
    Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box

    Applications

    Show applications key

    Our Abpromise guarantee covers the use of ab12075 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application notes

    IF: 1/1000. This dilution is determined by using paraformaldehyde fixed AFX transfected COS-7 cells.
    WB: 1/2000. This dilution is determined using human AFX expressed in transfected COS-7 cell extracts. Predicted molecular weight: 50 kDa. Staining of AFX is specifically inhibited by the AFX immunizing peptide.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Target

    Function

    Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.

    Tissue specificity

    Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.

    Involvement in disease

    Note=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.

    Sequence similarities

    Contains 1 fork-head DNA-binding domain.

    Post-translational
    modifications

    Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
    Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
    Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.

    Cellular localization

    Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.

    Target information above from: UniProt accessionP98177 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010).

    Information by UniProt

    Alternative names

    • AFX antibody
    • AFX1 antibody
    • Afxh antibody
    • ALL1-fused gene from X chromosome antibody
    • Fork head domain transcription factor AFX1 antibody
    • Forkhead box O4 antibody
    • Forkhead box protein O4 antibody
    • FOXO 4 antibody
    • Foxo4 antibody
    • FOXO4_HUMAN antibody
    • MGC117660 antibody
    • MGC120490 antibody
    • Mixed lineage leukemia, translocated to, 7 antibody
    • MLLT7 antibody
    • Myeloid lymphoid or mixed-lineage leukemia translocated to 7 antibody
    • Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 antibody
    • Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 antibody
    • Putative fork head domain transcription factor AFX1 antibody
    • RGD1561201 antibody
    see all

    Database links

    References for Anti-FOXO4 antibody (ab12075)

    ab12075 has not yet been referenced specifically in any publications.

    Publishing research using ab12075? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"