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ab16790 |
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Dear Dr Danielle Miller, Thank you very much for your answer with informations. Could you please tell me with the new Fox P3 antibodies kit, how many T regulatory cells are required for labeling? (the minimum required) I am interested to have the protocole for labeling for FACS analysis too. Thank you in advance. Best regards, Dr Tu Tran |
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ANSWER: |
The new rabbit antibody will only have been tested in ELISA and WB at the time of release. We do not have the facilities here for FACS. The goat antibody had been tested in FACS but unfortuantely we have run out of that particular batch. Details of how the goat antibody was used in FACS can be found in the reviews section of the online datasheet. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot using ab4728 at 1/500.
Lane 1: HEK 293 lysate with ab4728
Lane 2: HEK 293 lysate over expressing human FOXP3 with ab4728
Lane 3: HEK 293 lysate over expressing mouse FOXP3 with ab4728
Lane 4: HEK 293 lysate with secondary antibody control ab6721.
Secondary antibody: Goat polyclonal to Rabbit IgG (HRP) ab6721 1/5000.
Exposure time: 30 sec.
Expected molecular weight: 47kD
IHC image of FOXP3 staining in human tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4728, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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