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- Proteins and Peptides
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Product name
Anti-FOXP2 antibody
See all FOXP2 products (4) ...
Description
Goat polyclonal to FOXP2
Tested applications
WB, IHC-P, IHC-Fr, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Cow, Dog, Pig
Immunogen
Synthetic peptide: REIEEEPLSEDLE, corresponding to C terminal amino acids 703-715 of Human FOXP2.
C-REIEEEPLSEDLE
General notes
Principal Names: FOXP2 (forkhead box P2), SPCH1, CAGH44, TNRC10, speech and language disorder 1, trinucleotide repeat containing 10, forkhead/winged-helix transcription factor.
GenBank Accession Number: NP055306
LocusLink ID: 93986
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box >> FOXP
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 79.9 kDa).
IHC-P: Use at an assay dependent dilution. PubMed: 16901978
IHC-Fr: Use at an assay dependent dilution. PubMed: 20484636
ELISA
ELISA: 1/32000.ELISA: 1/32000.
Target
Function
Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Involved in neural mechanisms mediating the development of speech and language.
Tissue specificity
Isoform 1 and isoform 6 are expressed in adult and fetal brain, caudate nucleus and lung.
Involvement in disease
Defects in FOXP2 are the cause of speech-language disorder 1 (SPCH1) [MIM:602081]; also known as autosomal dominant speech and language disorder with orofacial dyspraxia. Affected individuals have a severe impairment in the selection and sequencing of fine orofacial movements, which are necessary for articulation. They also show deficits in several facets of language processing (such as the ability to break up words into their constituent phonemes) and grammatical skills.
Note=A chromosomal aberration involving FOXP2 is a cause of severe speech and language impairment. Translocation t(5;7)(q22;q31.2).
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain.
Developmental stage
Expressed in the brain at 15 and 22 weeks of gestation, with a pattern of strong cortical, basal ganglia, thalamic and cerebellar expression. Highly expressed in the head and tail of nucleus caudatus and putamen. Restricted expression within the globus pallidus, with high levels in the pars interna, which provides the principal source of output from the basal ganglia to the nucleus centrum medianum thalami (CM) and the major motor relay nuclei of the thalamus. In the thalamus, present in the CM and nucleus medialis dorsalis thalami. Lower levels are observed in the nuclei anterior thalami, dorsal and ventral, and the nucleus parafascicularis thalami. Expressed in the ventrobasal complex comprising the nucleus ventralis posterior lateralis/medialis. The ventral tier of the thalamus exhibits strong expression, including nuclei ventralis anterior, lateralis and posterior lateralis pars oralis. Also expressed in the nucleus subthalamicus bilaterally and in the nucleus ruber.
Domain
The leucine-zipper is required for dimerization and transcriptional repression.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO15409
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CAG repeat protein 44 antibody
- CAGH44 antibody
- DKFZp686H1726 antibody
see all
Database links
- Entrez Gene: 93986 Human
- Entrez Gene: 114142 Mouse
- Omim: 605317 Human
- SwissProt: O15409 Human
- SwissProt: P58463 Mouse
- SwissProt: P0CF24 Rat
- Unigene: 282787 Human
- Unigene: 332919 Mouse
see all
Anti-FOXP2 antibody images:
Western blot - FOXP2 antibody (ab1307)
Anti-FOXP2 antibody (ab1307) at 0.5 µg/ml + Whole tissue lysate from human cerebellum (35µg protein in RIPA buffer)
Predicted band size : 79.9 kDa
Immunohistochemistry (Frozen sections) - FOXP2 antibody (ab1307)
ab1307 staining FOXP2 in e12.5 mouse embryonic spinal cord tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with paraformaldehyde and then blocked with 10% serum for 30 minutes at 20°C followed by incubation with the primary antibody, at a 1/1000 dilution, for 16 hours at 4°C. The secondary antibody used was a donkey anti-sheep (and goat) IgG conjugated to Alexa Fluor® 594 (red) used at a 1/2000 dilution.
Image courtesy of Dr F Clotman by Abreview.
References for Anti-FOXP2 antibody (ab1307)
This product has been referenced in:
- Arellano JI et al. Development and distribution of neuronal cilia in mouse neocortex. J Comp Neurol 520:848-73 (2012). IHC-FoFr; Mouse.Read more (PubMed: 22020803) »
- Waclaw RR et al. Developmental origin of the neuronal subtypes that comprise the amygdalar fear circuit in the mouse. J Neurosci 30:6944-53 (2010). IHC-Fr; Mouse.Read more (PubMed: 20484636) »
See all 4 publications for this product
Publishing research using ab1307? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"