![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FOXP3 antibody [236A/E7] (ab20034) image](#ab20034_21.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FOXP3 antibody [236A/E7] (ab20034) image](#ab20034_11.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FOXP3 antibody [236A/E7] (ab20034) image](#FOXP3-Primary-antibodies-ab20034-4.jpg)
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Product name
Anti-FOXP3 antibody [236A/E7]
See all FOXP3 products (25) ...
Description
Mouse monoclonal [236A/E7] to FOXP3
Tested applications
Flow Cyt, IHC-P, IHC-Fr, WB, IFmore details
Cross reactivity
Reacts with
Human, Cynomolgus Monkey, Rhesus monkey
Immunogen
FOXP3 fusion protein.
Positive control
Normal tonsil.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
236A/E7
Myeloma
P3-NS1/1-Ag4-1
Isotype
IgG1
Research areas
Immunology >> Adaptive Immunity >> Regulatory T Cells
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box >> FOXP
Cell Biology >> Cell Cycle >> Cell Division >> Chromatid Cohesion
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FOXP3 antibody [236A/E7] (ab20034)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FOXP3 antibody [236A/E7] (ab20034) image](/ps/datasheet/Images/20/ab20034/ab20034_21.jpg)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FOXP3 antibody [236A/E7] (ab20034)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FOXP3 antibody [236A/E7] (ab20034) image](/ps/datasheet/Images/20/ab20034/ab20034_11.jpg)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FOXP3 antibody [236A/E7] (ab20034)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FOXP3 antibody [236A/E7] (ab20034) image](/ps/datasheet/images/20/ab20034/FOXP3-Primary-antibodies-ab20034-4.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab20034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
IHC-P: Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-Fr: Use a concentration of 10 µg/ml.
WB: Use a concentration of 4 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
IF: Use a concentration of 20 µg/ml.
Target
Function
Probable transcription factor. Plays a critical role in the control of immune response.
Involvement in disease
Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9BZS1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AIID antibody
- DIETER antibody
- Forkhead box P3 antibody
see all
Database links
- Entrez Gene: 50943 Human
- Omim: 300292 Human
- SwissProt: Q9BZS1 Human
- Unigene: 247700 Human
Anti-FOXP3 antibody [236A/E7] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FOXP3 antibody [236A/E7] (ab20034)
ab20034 used on paraffin embedded human tonsil sections.
Double staining: CD30 in red, FOXP3 in black, blue is haemotoxylin counterstaining of nuclei.
As expected FOXP3 stains a sub-population of T cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FOXP3 antibody [236A/E7] (ab20034)
ab20034 used on paraffin embedded human tonsil sections.
FOXP3 staining is black, blue is haemotoxylin counterstaining of nuclei.
As expected FOXP3 stains a sub-population of T cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FOXP3 antibody [236A/E7] (ab20034)
ab20034 staining FOXP3 in human colon tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval and then blocking with 10% serum for 2 hours at 21°C. The primary antibody was diluted 1/50 and incubated with sample for 2 hours at 21°C. An Alexa Fluor®488-conjugated rabbit polyclonal to mouse IgG was used undiluted as secondary antibody.
This image is a courtesy of Nicole Schechter
Immunohistochemistry (Frozen sections) - FOXP3 antibody [236A/E7] (ab20034)
![Immunohistochemistry (Frozen sections) - FOXP3 antibody [236A/E7] (ab20034)](/ps/datasheet/images/20/ab20034/FOXP3-Primary-antibodies-ab20034-6.jpg)
ab20034 staining FOXP3 in Human breast cancer tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone/methanol (1:2), permeabilized with TBS-TritonX-100 and blocked with 10% serum for 10 minutes at 20°C. Samples were incubated with primary antibody (1/50 in PBT (PBS/BSA/Tween)) for 30 minutes at 20°C. An undiluted HRP-conjugated rat anti-mouse/rabbit IgG polyclonal was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
References for Anti-FOXP3 antibody [236A/E7] (ab20034)
This product has been referenced in:
- Kuo CH et al. Concomitant active tuberculosis prolongs survival in non-small cell lung cancer: a study in a tuberculosis-endemic country. PLoS One 7:e33226 (2012). IHC-P; Human.Read more (PubMed: 22438899) »
- de Boer OJ et al. Spatial Differences in the Presence of FOXP3 and GranzymeB T Cells between the Intra- and Extravascular Compartments in Renal Allograft Vasculopathy. PLoS One 6:e18656 (2011). IHC-P; Human.Read more (PubMed: 21494640) »
See all 47 publications for this product
Publishing research using ab20034? Please let us know so that we can cite the reference in this datasheet
![FOXP3 antibody [236A/E7] for IHC-P in Human (20034)](/ps/Reviews/Images/ab4160_12023.jpg)
![FOXP3 antibody [236A/E7] for IHC-P in Human (20034)](/ps/Reviews/Images/ab11815_62409.jpg)
![FOXP3 antibody [236A/E7] for IHC-P in Human (20034)](/ps/datasheet/images/20/ab20034/FOXP3-Primary-antibodies-ab20034-1.jpg)
![FOXP3 antibody [236A/E7] for IHC-P in Human (20034)](/ps/datasheet/images/20/ab20034/FOXP3-Primary-antibodies-ab20034-2.jpg)
![FOXP3 antibody [236A/E7] for IHC-P in Human (20034)](/ps/datasheet/images/20/ab20034/FOXP3-Primary-antibodies-ab20034-3.jpg)
![FOXP3 antibody [236A/E7] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (20034)](/ps/datasheet/images/20/ab20034/FOXP3-Primary-antibodies-ab20034-8.jpg)
![FOXP3 antibody [236A/E7] for Immunohistochemistry (Frozen sections) in Human (20034)](/ps/datasheet/images/20/ab20034/FOXP3-Primary-antibodies-ab20034-5.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"