![Flow Cytometry - Anti-FOXP3 antibody [3G3] (ab99873) image](#FOXP3-Primary-antibodies-ab99873-1.jpg)
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Product name
Anti-FOXP3 antibody [3G3]
See all FOXP3 products (25) ...
Description
Mouse monoclonal [3G3] to FOXP3
Tested applications
WB, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Full-length His-tagged recombinant murine FOXP3
Epitope
The mouse monoclonal antibody 3G3 recognizes the N-terminal region of FOXP3.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
ab99873 was purified from ascites by protein A affinity chromatography
Clonality
Monoclonal
Clone number
3G3
Isotype
IgG1
Research areas
Immunology >> Adaptive Immunity >> Regulatory T Cells
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box >> FOXP
Cell Biology >> Cell Cycle >> Cell Division >> Chromatid Cohesion
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Flow Cytometry - Anti-FOXP3 antibody [3G3] (ab99873)
![Flow Cytometry - Anti-FOXP3 antibody [3G3] (ab99873) image](/ps/datasheet/images/99/ab99873/FOXP3-Primary-antibodies-ab99873-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab99873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2 µg/ml. Predicted molecular weight: 47 kDa.
Flow Cyt: Use a concentration of 2 - 10 µg/ml. Optimal concentration for peripheral blood is 3 µg/ml.
Target
Function
Probable transcription factor. Plays a critical role in the control of immune response.
Involvement in disease
Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9BZS1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AIID antibody
- DIETER antibody
- Forkhead box P3 antibody
see all
Database links
- Entrez Gene: 50943 Human
- Entrez Gene: 20371 Mouse
- Omim: 300292 Human
- SwissProt: Q9BZS1 Human
- SwissProt: Q99JB6 Mouse
- Unigene: 247700 Human
- Unigene: 182291 Mouse
Anti-FOXP3 antibody [3G3] images:
Flow Cytometry - Anti-FOXP3 antibody [3G3] (ab99873)
ab99873 performing intracellular staining of human peripheral blood cells (gated on CD4+ cells).
References for Anti-FOXP3 antibody [3G3] (ab99873)
This product has been referenced in:
- Gavin MA et al. Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development. Proc Natl Acad Sci U S A 103:6659-64 (2006).Read more (PubMed: 16617117) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"