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ab14151 |
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ab14151 |
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ab10563 has been referenced in 19 publications.
Publishing research using ab10563? Please let us know so that we can cite the reference in this datasheet
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Immunofluorescent imaging of human cells (U2OS) with ab10563 (5 ug/ml) confirms the specificity of this anti-FOXP3 antibody, which recognises nuclear signal only; with no background cytoplasmic or nucleolar staining.
IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour). Primary antibody used at 5ug/ml in 5% milk / 0.2% TWEEN for 1 hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees C. Nuclei were stained with Hoechst stain.
Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK
FoxP3 immunostaining on a frozen section from human tonsil. Original magnification x100. Nuclear staining was observed.
Cigdem Atayar
Lanes 1 - 6 : Anti-FOXP3 antibody (ab10563) at 2 µg/ml This antibody recognises a single clean band in a HEK293 cell lysate that overexpresses human FOXP3. The band is not seen in untransfected cells and is blocked by the immunising peptide.
Lane 7 :
Lane 1 : 20µg HEK 293 lysate
Lane 2 : 20µg HEK 293 lysate over expressing human FOXP3
Lane 3 : 20µg HEK 293 lysate over expressing mouse FOXP3
Lane 4 : 20µg HEK 293 lysate with
Lane 5 : 20µg HEK 293 lysate over expressing human FOXP3 with
Lane 6 : 20µg HEK 293 lysate over expressing mouse FOXP3 with
Lane 7 : 20µg HEK 293 lysate
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 0.2 µg/ml
Performed under reducing conditions.
Predicted band size : 47.2 kDa
Observed band size : 47 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
2
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