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Products:Cell Biology >> Cell Cycle >> Cell Division >> Chromatid Cohesion
Overview
Product name
Anti-FOXP3 antibody
See all FOXP3 products (28) ...
Description
Rabbit polyclonal to FOXP3
Specificity
This antibody recognises a single band representing human FOXP3 in a HEK293 lysate overexpressing human FOXP3.
Tested applications
WB, IHC-P, Flow Cytmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse
Immunogen
Synthetic peptide derived from residues 1 - 100 of Human FOXP3.
.
Positive control
This antibody gave a positive signal in Hek293 lysate over expressing FoxP3 and Human tonsil.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Adaptive Immunity >> Regulatory T Cells
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box >> FOXP
Cell Biology >> Cell Cycle >> Cell Division >> Chromatid Cohesion
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt: Use at an assay dependent dilution.
Target
Function
Probable transcription factor. Plays a critical role in the control of immune response.
Involvement in disease
Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9BZS1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AIID antibody
- DIETER antibody
- Forkhead box P3 antibody
see all
Database links
- Entrez Gene: 50943 Human
- Omim: 300292 Human
- SwissProt: Q9BZS1 Human
- Unigene: 247700 Human
Anti-FOXP3 antibody images:
- FOXP3 antibody (ab4728)
Western blot using ab4728 at 1/500.
Lane 1: HEK 293 lysate with ab4728
Lane 2: HEK 293 lysate over expressing human FOXP3 with ab4728
Lane 3: HEK 293 lysate over expressing mouse FOXP3 with ab4728
Lane 4: HEK 293 lysate with secondary antibody control ab6721.
Secondary antibody: Goat polyclonal to Rabbit IgG (HRP) ab6721 1/5000.
Exposure time: 30 sec.
Expected molecular weight: 47kD
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FOXP3 antibody (ab4728)
IHC image of FOXP3 staining in human tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4728, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
References for Anti-FOXP3 antibody (ab4728)
This product has been referenced in:
- Popmihajlov Z & Smith KA Negative feedback regulation of T cells via interleukin-2 and FOXP3 reciprocity. PLoS ONE 3:e1581 (2008). Flow Cyt; Human.Read more (PubMed: 18324310) »
- Oh Uet al. Reduced Foxp3 Protein Expression Is Associated with Inflammatory Disease during Human T Lymphotropic Virus Type 1 Infection. J Infect Dis 193:1557-66 (2006).Read more (PubMed: 16652285) »
See all 3 publications for this product
Publishing research using ab4728? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"