|
|
|
|
- FOXP3 antibody (ab4728)
(enlarge)
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FOXP3 antibody (ab4728)
(enlarge)
|
|
|
|
|
Product Name
|
FOXP3 antibody
|
| |
See all FOXP3 antibodies (19)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to FOXP3
|
|
Immunogen
|
Synthetic peptide derived from residues 1 - 100 of Human FOXP3.
.
|
|
Reacts with
|
Hu
|
|
Does not react with
(species key)
|
Ms
|
|
Specificity
|
This antibody recognises a single band representing human FOXP3 in a HEK293 lysate overexpressing human FOXP3.
|
|
Tested applications
(see key)
|
Flow Cyt, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IHC-P: Use at a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Immunology >> Adaptive Immunity >> Regulatory T Cells
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box >> FOXP
Cell Biology >> Cell Cycle >> Cell Division >> Chromatid Cohesion
|
|
Relevance
|
FOXP3 (a 431 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis.
FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, at low level in CD4 positive/CD25 negative cells, and is absent in CD4 negative/CD8 positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
Batch dependent within range: 0.40 - 0.40 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FOXP3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for FOXP3 antibody images, references, products related to ab4728 and other tools.
|
| |
FOXP3 antibody images:
|
 - FOXP3 antibody (ab4728)
Western blot using ab4728 at 1/500.
Lane 1: HEK 293 lysate with ab4728
Lane 2: HEK 293 lysate over expressing human FOXP3 with ab4728
Lane 3: HEK 293 lysate over expressing mouse FOXP3 with ab4728
Lane 4: HEK 293 lysate with secondary antibody control ab6721.
Secondary antibody: Goat polyclonal to Rabbit IgG (HRP) ab6721 1/5000.
Exposure time: 30 sec.
Expected molecular weight: 47kD
|
| |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FOXP3 antibody (ab4728)
IHC image of FOXP3 staining in human tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4728, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
|
| |
References for FOXP3 antibody (ab4728)
|
|
This product has been used in: (two most recent references)
Popmihajlov Z & Smith KA Negative feedback regulation of T cells via interleukin-2 and FOXP3 reciprocity. PLoS ONE 3:e1581 (2008). Flow Cyt; Human. PubMed: 18324310
Oh U et al. Reduced Foxp3 Protein Expression Is Associated with Inflammatory Disease during Human T Lymphotropic Virus Type 1 Infection. J Infect Dis 193:1557-66 (2006). PubMed: 16652285
See all 3 publication references for this product.
If you publish research using ab4728 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to FOXP3
|
| |
FOXP3 antibody - more information
|
Customer reviews (feedback) regarding FOXP3 antibody |
Customer FAQs regarding FOXP3 antibody |
Protocols for FOXP3 antibody |
Price and availability of products related to FOXP3 antibody |
| |
FOXP3 antibody - related products:
|
|
|
| |
FOXP3 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about FOXP3 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
