Anti-FPR1 antibody (ab113531)
Key features and details
- Rabbit polyclonal to FPR1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FPR1 antibody -
Description
Rabbit polyclonal to FPR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide conjugated to KLH, from an internal region of Human FPR1.
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Positive control
- Human spleen tissue; K562 cell extracts; MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 49.1% PBS
PBS without Mg2+, Ca2+ -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 38 kDa.
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IHC-P |
Use a concentration of 5 µg/ml.
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ICC/IF | (1) |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 38 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
ICC/IF
1/100 - 1/500. |
Target
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Function
High affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. -
Tissue specificity
Neutrophils. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsPhosphorylated; which is necessary for desensitization. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2357 Human
- Omim: 136537 Human
- SwissProt: P21462 Human
- Unigene: 753 Human
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Alternative names
- fMet Leu Phe receptor antibody
- fMet-Leu-Phe receptor antibody
- FMLP antibody
see all
Images
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All lanes : Anti-FPR1 antibody (ab113531) at 1/500 dilution
Lane 1 : K562 cell extract
Lane 2 : K562 cell extract with immunizing peptide
Predicted band size: 38 kDa -
ab113531 at 5ug/ml staining FPR1 in Formalin-Fixed, Paraffin-Embedded Human spleen tissue by Immunohistochemistry.
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ab113531 at a 1/100 dilution staining FPR1 in Human MCF7 cells by Immunofluorescence. The cells on the right were incubated with the immunizing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab113531 has been referenced in 13 publications.
- Fischer A et al. Neutrophils direct preexisting matrix to initiate repair in damaged tissues. Nat Immunol 23:518-531 (2022). PubMed: 35354953
- Chen C et al. The Role of Formyl Peptide Receptor 1 in Uterine Contraction During Parturition. Front Pharmacol 12:696697 (2021). PubMed: 34393780
- Pouwels SD et al. Acute cigarette smoke-induced eQTL affects formyl peptide receptor expression and lung function. Respirology 26:233-240 (2021). PubMed: 33078507
- Li BB et al. Cell invasion in digital microfluidic microgel systems. Sci Adv 6:eaba9589 (2020). PubMed: 32832633
- Gorrasi A et al. New Pieces in the Puzzle of uPAR Role in Cell Migration Mechanisms. Cells 9:N/A (2020). PubMed: 33255171