Anti-FRMD4A antibody (ab122475)
Key features and details
- Rabbit polyclonal to FRMD4A
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FRMD4A antibody -
Description
Rabbit polyclonal to FRMD4A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: EGAHDKGAGR AAVSDELRQW YQRSTASHKE HSRLSHTSST SSDSGSQYST SSQSTFVAHS RVTRMPQMCK ATSA, corresponding to amino acids 912-985 of Human FRMD4A.
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Positive control
- IHC: Human testis, kidney, lymphoid and cerebral cortex tissue. ICC/IF: Human cell line U-2 OS shows
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Sequence similarities
Contains 1 FERM domain. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 55691 Human
- SwissProt: Q9P2Q2 Human
- Unigene: 330463 Human
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Alternative names
- bA295P9.4 antibody
- FERM domain containing 4A antibody
- FERM domain-containing protein 4A antibody
see all
Images
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ab122475 at 1/50 dilution staining FRMD4A in paraffin-embedded Human lymphoid tissue by Immunohistochemistry.
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and golgi apparatus. Recommended concentration of ab122475 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab122475 at 1/50 dilution staining FRMD4A in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
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ab122475 at 1/50 dilution staining FRMD4A in paraffin-embedded Human kidney tissue by Immunohistochemistry.
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ab122475 at 1/50 dilution staining FRMD4A in paraffin-embedded Human testis tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab122475 has been referenced in 1 publication.
- Fan Y et al. Increased expression of FERM domain-containing 4A protein is closely associated with the development of rectal cancer. Exp Ther Med 11:421-426 (2016). PubMed: 26893625