The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: use neat for 10 min at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate pH 6.0 for 10 min and then cool at room temperature for 20 min.
Staining pattern: Cytoplasmic
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceFollicle stimulating hormone enables ovarian folliculogenesis to the antral follicle stage and is essential for Sertoli cell proliferation and maintenance of sperm quality in the testis. Members of the pituitary glycoprotein hormone family, of which FSH is one (see also luteinizing hormone, chorionic gonadotropin, and thyroid stimulating hormone), consist of a shared alpha chain and a beta chain encoded by a separate gene. The FSHB gene encodes the beta subunit of follicle stimulating hormone.