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- Proteins and Peptides
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Product name
Anti-FXYD1 antibody
See all FXYD1 products (2) ...
Description
Rabbit polyclonal to FXYD1
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Cow, Dog, Macaque Monkey
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human FXYD1.
(Peptide available as ab881 61.)
Positive control
This antibody gave a positive signal in the following tissue lysates: Human Brain; Human Brain Membrane; Human Heart; Human Skeletal Muscle
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Cardiovascular >> Heart >> Contractility >> Inotropics
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
IHC-P: Use at an assay dependent concentration.
Target
Relevance
PLM (FXYD1)is a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. PLM may be phosphorylated by several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity.
Cellular localization
Membrane; single-pass type I membrane protein
Alternative names
- FXYD domain containing ion transport regulator 1 antibody
- Phospholemman antibody
- PLM antibody
Database links
- Entrez Gene: 476487 Dog
- Entrez Gene: 5348 Human
- Entrez Gene: 56188 Mouse
- Entrez Gene: 58971 Rat
- Omim: 602359 Human
- SwissProt: P56513 Dog
- SwissProt: O00168 Human
- SwissProt: Q9Z239 Mouse
see all
Anti-FXYD1 antibody images:
Western blot - FXYD1 antibody (ab76597)
All lanes : Anti-FXYD1 antibody (ab76597) at 1 µg/ml
Lane 1 :
Lane 2 :
Lane 3 :
Lane 4 :
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 10 kDa
Observed band size : 10 kDa
Exposure time : 5 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FXYD1 antibody (ab76597)
ab76597 at a 1/100 dilution, staining mouse FXYD1 in heart and muscle tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.
References for Anti-FXYD1 antibody (ab76597)
ab76597 has not yet been referenced specifically in any publications.
Publishing research using ab76597? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"