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Product name
Anti-FZD3 antibody
See all FZD3 products (3) ...
Description
Mouse monoclonal to FZD3
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment, corresponding to amino acids 55-158 of Human FZD3
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> Surface Molecules
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
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Western blot - FZD3 antibody (ab56331)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Tissue specificity
Widely expressed. Relatively high expression in the CNS, including regions of the limbic system, in kidney, pancreas, skeletal muscle, uterus and testis.
Sequence similarities
Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain.
Domain
Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands.
Cellular localization
Membrane. Cell membrane.
Target information above from: UniProt accessionQ9NPG1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Frizzled 3 antibody
- Frizzled homolog 3 (Drosophila) antibody
- Frizzled homolog 3 antibody
see all
Database links
- Entrez Gene: 7976 Human
- Omim: 606143 Human
- SwissProt: Q9NPG1 Human
- Unigene: 40735 Human
Anti-FZD3 antibody images:
Western blot - FZD3 antibody (ab56331)
Western blot against tagged recombinant protein immunogen using ab56331 FZD3 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
References for Anti-FZD3 antibody (ab56331)
This product has been referenced in:
- Valastyan S et al. A pleiotropically acting microRNA, miR-31, inhibits breast cancer metastasis. Cell 137:1032-46 (2009). WB; Human.Read more (PubMed: 19524507) »
See 1 publication for this product
Publishing research using ab56331? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"