You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameFactor B protein (Tagged-His Tag)See all Factor B proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 77-764 of Human Factor B with N terminal His tag; MWt 91kDa (UniProt P00751).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 100 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesRSTGSWSTLKTQDQKTVRKAECRAIHCPRPHDFENGEYWP RSPYYNVSDE ISFHCYDGYTLRGSANRTCQVNGRWSGQ TAICDNGAGYCSNPGIPIGTRK VGSQYRLEDSVTYHCS RGLTLRGSQRRTCQEGGSWSGTEPSCQDSFMYDT PQEV AEAFLSSLTETIEGVDAEDGHGPGEQQKRKIVLDPSGSMN IYLVLD GSDSIGASNFTGAKKCLVNLIEKVASYGVKPR YGLVTYATYPKIWVKVSE ADSSNADWVTKQLNEINYED HKLKSGTNTKKALQAVYSMMSWPDDVPPEG WNRTRHVI ILMTDGLHNMGGDPITVIDEIRDLLYIGKDRKNPREDYLD VY VFGVGPLVNQVNINALASKKDNEQHVFKVKDMENLE DVFYQMIDESQSLS LCGMVWEHRKGTDYHKQPWQAKIS VIRPSKGHESCMGAVVSEYFVLTAAH CFTVDDKEHSIK VSVGGEKRDLEIEVVLFHPNYNINGKKEAGIPEFYDYD VALIKLKNKLKYGQTIRPICLPCTEGTTRALRLPPTTTCQ QQKEELLPAQ DIKALFVSEEEKKLTRKEVYIKNGDKKG SCERDAQYAPGYDKVKDISEVV TPRFLCTGGVSPYADP NTCRGDSGGPLIVHKRSRFIQVGVISWGVVDVCK NQKR QKQVPAHARDFHINLFQVLPWLKEKLQDEDLGFL
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91670 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        AHUS4B factor properdinBF
        BFDC3 proacceleratorC3 proactivatorC3/C5 convertaseCFABCFAB_HUMANCFBComplement Factor BComplement factor B Bb fragmentFBFBI12GBGGlycine rich beta glycoproteinGlycine-rich beta glycoproteinH2 BfH2BFPBF2Properdin factor B
      see all
  • FunctionFactor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.
  • Involvement in diseaseDefects in CFB are a cause of susceptibility to hemolytic uremic syndrome atypical type 4 (AHUS4) [MIM:612924]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 3 Sushi (CCP/SCR) domains.
    Contains 1 VWFA domain.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P00751 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Factor B protein (Tagged-His Tag) images

    • SDS-PAGE - Factor B protein (Tagged-His Tag) (ab91670)
      SDS-PAGE - Factor B protein (Tagged-His Tag) (ab91670)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for Factor B protein (Tagged-His Tag) (ab91670)

    ab91670 has not yet been referenced specifically in any publications.

    Publishing research using ab91670 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab91670 Submit a Question for ab91670

    There are currently no Abreviews or Questions for ab91670.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"