Overview
- Product nameAnti-Factor IX antibody [9D]See all Factor IX primary antibodies ...
- DescriptionMouse monoclonal [9D] to Factor IX
- Tested applicationsELISA, Sandwich ELISA, WB more details
- Species reactivityReacts with: Human
- Immunogen
Full length native protein: Human Factor IX
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.01% Sodium Azide
Constituents: 150mM Sodium chloride, 10mM Sodium phosphate. pH 7.4 -
Concentration information loading... - Purification notesPurified from ascites by gel filtration and anion exchange chromatography.
- Clonality Monoclonal
- Clone number9D
- MyelomaNS1
- IsotypeIgG1
- Light chain typeunknown
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab21045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: Use at an assay dependent dilution. |
| Sandwich ELISA | sELISA: Use a concentration of 5 µg/ml. For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to Factor IX (ab23335) as Detection. |
| WB | WB: Use at an assay dependent dilution. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). This antibody recognizes an epitope on the light chain. On Western blot using reducing conditions, the molecular weight of the light chain is 23kDa. Dilution optimised using Chromogenic detection. On Western blot using non-reducing conditions, the molecular weight of the intact Factor IX is 57kDa. |
Target
- FunctionFactor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
- Tissue specificitySynthesized primarily in the liver and secreted in plasma.
- Involvement in diseaseDefects in F9 are the cause of recessive X-linked hemophilia B (HEMB) [MIM:306900]; also known as Christmas disease.
Note=Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide.
Defects in F9 are the cause of thrombophilia due to factor IX defect (THR-FIX) [MIM:300807]. A hemostatic disorder characterized by a tendency to thrombosis. - Sequence similaritiesBelongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. - DomainCalcium binds to the gamma-carboxyglutamic acid (Gla) residues and, with stronger affinity, to another site, beyond the Gla domain.
- Post-translational
modificationsActivated by factor XIa, which excises the activation peptide.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. - Cellular localizationSecreted.
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Database links
- Entrez Gene: 2158 Human
- Omim: 300746 Human
- SwissProt: P00740 Human
- Unigene: 522798 Human
Target information above from: UniProt accession
P00740
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Christmas Disease antibodyChristmas factor antibodyCoagulant factor IX antibody
- Coagulation factor 9 antibodyCoagulation factor IX (plasma thromboplastic component) antibodyCoagulation factor IX antibodyCoagulation factor IXa heavy chain antibodyF9 antibodyFA9_HUMAN antibodyFactor 9 antibodyFactor IX Deficiency antibodyFactor9 antibodyFactorIX antibodyFIX antibodyGLA domain antibodyHaemophilia B antibodyHEMB antibodyMGC129641 antibodyMGC129642 antibodyP19 antibodyPlasma thromboplastic component antibodyPlasma thromboplastin component antibodyPTC antibodyTruncated coagulation factor IX antibody
see all
Anti-Factor IX antibody [9D] images
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![Sandwich ELISA - Factor IX antibody [9D] (ab21045)](http://a.abcam.com/ps/datasheet/images/21/ab21045/Factor-IX-Primary-antibodies-ab21045-3.jpg)
Sandwich ELISA - Factor IX antibody [9D] (ab21045)Standard Curve for Factor IX (Analyte: Factor IX protein (ab62544)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [9D] to Factor IX (ab21045) at 5µg/ml and Detector Antibody Rabbit polyclonal to Factor IX (ab23335) at 0.5µg/ml. -
![Western blot - Factor IX antibody [9D] (ab21045)](http://a.abcam.com/ps/datasheet/images/21/ab21045/Factor-IX-Primary-antibodies-ab21045-2.jpg)
Western blot - Factor IX antibody [9D] (ab21045)Anti-Factor IX antibody [9D] (ab21045) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 52 kDa
Observed band size : 52 kDa
Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
Protocols
References for Anti-Factor IX antibody [9D] (ab21045)
ab21045 has not yet been referenced specifically in any publications.
