Overview

  • Product nameAnti-Factor IX antibody
    See all Factor IX primary antibodies
  • Description
    Rabbit polyclonal to Factor IX
  • Tested applicationsIHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog, Pig, Monkey
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 217 and 423 of Human Factor IX (P00740).

  • Positive control
    • 293T whole cell lysate IHC-P: Human heart FFPE tissue sections

Properties

Applications

Our Abpromise guarantee covers the use of ab97619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.

Target

  • FunctionFactor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
  • Tissue specificitySynthesized primarily in the liver and secreted in plasma.
  • Involvement in diseaseDefects in F9 are the cause of recessive X-linked hemophilia B (HEMB) [MIM:306900]; also known as Christmas disease.
    Note=Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide.
    Defects in F9 are the cause of thrombophilia due to factor IX defect (THR-FIX) [MIM:300807]. A hemostatic disorder characterized by a tendency to thrombosis.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • DomainCalcium binds to the gamma-carboxyglutamic acid (Gla) residues and, with stronger affinity, to another site, beyond the Gla domain.
  • Post-translational
    modifications
    Activated by factor XIa, which excises the activation peptide.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Christmas Disease antibody
    • Christmas factor antibody
    • Coagulant factor IX antibody
    • Coagulation factor 9 antibody
    • Coagulation factor IX antibody
    • Coagulation factor IXa heavy chain antibody
    • F9 antibody
    • FA9_HUMAN antibody
    • Factor 9 antibody
    • Factor IX Deficiency antibody
    • FactorIX antibody
    • FIX antibody
    • Haemophilia B antibody
    • HEMB antibody
    • MGC129641 antibody
    • MGC129642 antibody
    • P19 antibody
    • Plasma Thromboplastic Component antibody
    • Plasma thromboplastin component antibody
    • PTC antibody
    see all

Anti-Factor IX antibody images

  • IHC image of Factor IX staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97619, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-Factor IX antibody (ab97619) at 1/1000 dilution + 293T whole cells lysate at 30 µg

    Predicted band size : 52 kDa
    10% SDS Page

References for Anti-Factor IX antibody (ab97619)

ab97619 has not yet been referenced specifically in any publications.

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