Anti-Factor IX antibody (ab97619)

Overview

• Product nameAnti-Factor IX antibodySee all Factor IX primary antibodies ...
• Description
  Rabbit polyclonal to Factor IX
• Tested applicationsIHC-P, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Dog, Pig, Monkey
• Immunogen

  Recombinant protein fragment corresponding to a region within amino acids 217 and 423 of Human Factor IX (P00740).

• Positive control293T whole cell lysate IHC-P: Human heart FFPE tissue sections

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
  Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cardiovascular
  • Blood
  • Other

  • Cardiovascular
  • Blood
  • Coagulation
  • Intrinsic

  • Immunology
  • Innate Immunity
  • Complement
  • Classical Pathway

Applications

Target

• FunctionFactor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
• Tissue specificitySynthesized primarily in the liver and secreted in plasma.
• Involvement in diseaseDefects in F9 are the cause of recessive X-linked hemophilia B (HEMB) [MIM:306900]; also known as Christmas disease.
  Note=Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide.
  Defects in F9 are the cause of thrombophilia due to factor IX defect (THR-FIX) [MIM:300807]. A hemostatic disorder characterized by a tendency to thrombosis.
• Sequence similaritiesBelongs to the peptidase S1 family.
  Contains 2 EGF-like domains.
  Contains 1 Gla (gamma-carboxy-glutamate) domain.
  Contains 1 peptidase S1 domain.
• DomainCalcium binds to the gamma-carboxyglutamic acid (Gla) residues and, with stronger affinity, to another site, beyond the Gla domain.
• Post-translational
  modificationsActivated by factor XIa, which excises the activation peptide.
  The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
• Cellular localizationSecreted.

Target information above from: UniProt accession P00740 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2158 Human
  • Omim: 300746 Human
  • SwissProt: P00740 Human
  • Unigene: 522798 Human

• Alternative names
  Christmas Disease antibodyChristmas factor antibodyCoagulant factor IX antibody

  Coagulation factor 9 antibodyCoagulation factor IX antibodyCoagulation factor IXa heavy chain antibodyF9 antibodyFA9_HUMAN antibodyFactor 9 antibodyFactor IX Deficiency antibodyFactorIX antibodyFIX antibodyHaemophilia B antibodyHEMB antibodyMGC129641 antibodyMGC129642 antibodyP19 antibodyPlasma Thromboplastic Component antibodyPlasma thromboplastin component antibodyPTC antibody

  see all

Anti-Factor IX antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor IX antibody (ab97619)

  IHC image of Factor IX staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97619, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Western blot - Factor IX antibody (ab97619)
  Anti-Factor IX antibody (ab97619) at 1/1000 dilution + 293T whole cells lysate at 30 µg
  
  Predicted band size : 52 kDa
  10% SDS Page