Anti-Factor IX antibody [EPR6187] (ab124815)
- Product nameAnti-Factor IX antibody [EPR6187]See all Factor IX primary antibodies ...
- DescriptionRabbit monoclonal [EPR6187] to Factor IX
- Tested applicationsWB, Flow Cyt, ICC/IF more details
- Species reactivityReacts with: Human
Does not react withMouse, Rat
Synthetic peptide, corresponding to residues in Human Factor IX.
- Positive controlHepG2 and Human plasma lysates; Permeabilized HepG2 cells.
- General notesProduced under U.S. Patent No. 5,675,063
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Dissociation constant (Kd) Kd = 2.74 x 10-11 M
- Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR6187
- Research Areas
Our Abpromise guarantee covers the use of ab124815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/1000 - 1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 52 kDa).|
|Flow Cyt||Flow Cyt: 1/10 - 1/100.|
|ICC/IF||ICC/IF: 1/50 - 1/100.|
- FunctionFactor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
- Tissue specificitySynthesized primarily in the liver and secreted in plasma.
- Involvement in diseaseDefects in F9 are the cause of recessive X-linked hemophilia B (HEMB) [MIM:306900]; also known as Christmas disease.
Note=Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide.
Defects in F9 are the cause of thrombophilia due to factor IX defect (THR-FIX) [MIM:300807]. A hemostatic disorder characterized by a tendency to thrombosis.
- Sequence similaritiesBelongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain.
- DomainCalcium binds to the gamma-carboxyglutamic acid (Gla) residues and, with stronger affinity, to another site, beyond the Gla domain.
modificationsActivated by factor XIa, which excises the activation peptide.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
- Cellular localizationSecreted.
- Christmas Disease antibodyChristmas factor antibodyCoagulant factor IX antibody
- Coagulation factor 9 antibodyCoagulation factor IX antibodyCoagulation factor IXa heavy chain antibodyF9 antibodyFA9_HUMAN antibodyFactor 9 antibodyFactor IX Deficiency antibodyFactorIX antibodyFIX antibodyHaemophilia B antibodyHEMB antibodyMGC129641 antibodyMGC129642 antibodyP19 antibodyPlasma Thromboplastic Component antibodyPlasma thromboplastin component antibodyPTC antibody
Anti-Factor IX antibody [EPR6187] images
All lanes : Anti-Factor IX antibody [EPR6187] (ab124815) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Human plasma
Lysates/proteins at 10 µg per lane.
Predicted band size : 52 kDa
ab124815, at 1/10 dilution, staining Factor IX in permeabilized HepG2 cells (Red) compared to a nonspecific negative control antibody (Green) by Flow Cytometry.
Equilibrium disassociation constant (KD)
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References for Anti-Factor IX antibody [EPR6187] (ab124815)
ab124815 has not yet been referenced specifically in any publications.