Anti-Factor IX antibody [EPR6187] (ab124815)

Overview

• Product nameAnti-Factor IX antibody [EPR6187]See all Factor IX primary antibodies ...
• Description
  Rabbit monoclonal [EPR6187] to Factor IX
• Tested applicationsWB, Flow Cyt, ICC/IF more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  Synthetic peptide, corresponding to residues in Human Factor IX.

• Positive controlHepG2 and Human plasma lysates; Permeabilized HepG2 cells.
• General notesProduced under U.S. Patent No. 5,675,063

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Dissociation constant (Kd) Kd = 2.74 x 10^-11 M
• Storage bufferpH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR6187
• IsotypeIgG
• Research Areas
  • Cardiovascular
  • Blood
  • Other

  • Cardiovascular
  • Blood
  • Coagulation
  • Intrinsic

  • Immunology
  • Innate Immunity
  • Complement
  • Classical Pathway

Applications

Target

• FunctionFactor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
• Tissue specificitySynthesized primarily in the liver and secreted in plasma.
• Involvement in diseaseDefects in F9 are the cause of recessive X-linked hemophilia B (HEMB) [MIM:306900]; also known as Christmas disease.
  Note=Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide.
  Defects in F9 are the cause of thrombophilia due to factor IX defect (THR-FIX) [MIM:300807]. A hemostatic disorder characterized by a tendency to thrombosis.
• Sequence similaritiesBelongs to the peptidase S1 family.
  Contains 2 EGF-like domains.
  Contains 1 Gla (gamma-carboxy-glutamate) domain.
  Contains 1 peptidase S1 domain.
• DomainCalcium binds to the gamma-carboxyglutamic acid (Gla) residues and, with stronger affinity, to another site, beyond the Gla domain.
• Post-translational
  modificationsActivated by factor XIa, which excises the activation peptide.
  The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
• Cellular localizationSecreted.

Target information above from: UniProt accession P00740 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2158 Human
  • Omim: 300746 Human
  • SwissProt: P00740 Human
  • Unigene: 522798 Human

• Alternative names
  Christmas Disease antibodyChristmas factor antibodyCoagulant factor IX antibody

  Coagulation factor 9 antibodyCoagulation factor IX antibodyCoagulation factor IXa heavy chain antibodyF9 antibodyFA9_HUMAN antibodyFactor 9 antibodyFactor IX Deficiency antibodyFactorIX antibodyFIX antibodyHaemophilia B antibodyHEMB antibodyMGC129641 antibodyMGC129642 antibodyP19 antibodyPlasma Thromboplastic Component antibodyPlasma thromboplastin component antibodyPTC antibody

  see all

Anti-Factor IX antibody [EPR6187] images

• 

  Western blot - Anti-Factor IX antibody [EPR6187] (ab124815)
  All lanes : Anti-Factor IX antibody [EPR6187] (ab124815) at 1/1000 dilution
  
  Lane 1 : HepG2 cell lysate
  Lane 2 : Human plasma
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 52 kDa
  
• 

  Flow Cytometry - Anti-Factor IX antibody [EPR6187] (ab124815)
  ab124815, at 1/10 dilution, staining Factor IX in permeabilized HepG2 cells (Red) compared to a nonspecific negative control antibody (Green) by Flow Cytometry.
• 

  Other-Anti-Factor IX antibody [EPR6187](ab124815)
  Equilibrium disassociation constant (K_D)
  Learn more about K_D
  
  Click here to learn more about K_D