Factor VII Human ELISA Kit (ab108829)
- Product nameFactor VII Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Saliva, Serum, Plasma
- Assay typeSandwich
- Sensitivity= 6 ng/ml
- Range6.25 ng/ml - 200 ng/ml
- Assay time4h 00m
- Species reactivityReacts with: Human
- Product overview
Factor VII (FVII) is a vitamin K-dependent plasma glycoprotein that is synthesized in the liver and circulates in blood as a single-chain inactive zymogen with a molecular mass of 50 kDa. Upon tissue damage and vascular injury, the cell surface receptor and cofactor tissue factor binds and allosterically activates FVII to its active form, FVIIa. The tissue factor/FVIIa complex catalyzes the conversion of both Factor IX to Factor IXa and Factor X to Factor Xa to initiate coagulation via the extrinsic pathway. Very low levels of FVII are associated with severe coagulation disorders. Elevated plasma levels of FVII coagulant activity constitute an independent risk factor for fatal outcomes of coronary heart disease in middle-aged men.
ab108829 Factor VII (FVII) Human ELISA kit is designed for detection of Human Factor VII in plasma, serum, saliva, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures FVII in less than 4 hours. A monoclonal antibody specific for FVII has been pre-coated onto a 96-well microplate with removable strips. FVII in standards and samples is sandwiched by the immobilized antibody and the biotinylated monoclonal antibody specific for FVII, which is recognized by a streptavidinperoxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Factor VII Antibody 1 x 140µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Factor VII Standard 1 unit Human Factor VII Microplate 1 x 96 units Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml
- Research Areas
- Cf7Coagulation factor VIICoagulation factor VII (serum prothrombin conversion accelerator)
- Eptacog alfaF7 deficiencyFactor VII deficiencyFVIIFVII coagulation proteinHypoproconvertinemiaProconvertinSerum prothrombin conversion acceleratorSPCA
Our Abpromise guarantee covers the use of ab108829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Factor VII Human ELISA Kit (ab108829)
ab108829 has not yet been referenced specifically in any publications.