Factor X Human ELISA kit (ab108832)
- Product nameFactor X Human ELISA kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
- Assay typeSandwich
- Sensitivity= 1.5 ng/ml
- Range1.563 ng/ml - 100 ng/ml
- Assay time4h 00m
- Species reactivityReacts with: Human
- Product overview
Factor X is a plasma serine protease zymogen involved in the blood coagulation cascade. Factor X is purified from plasma as a two-chain protein consisting of a 45 kDa heavy chain and a 17 kDa light chain. The Factor X heavy chain is cleaved during coagulation by several different proteases including the intrinsic Xase complex, the Factor X-activating enzyme from Russell’s viper venom (RVV) and trypsin, and also by extrinsic (tissue factor/factor VIIa) pathway to give an active enzyme FXa. FXa as the activator of prothrombin occupies a central position linking the two blood coagulation pathways.
ab108832 Factor X Human ELISA (Enzyme-Linked Immunosorbent Assay) is designed for detection of human factor X in plasma, serum, milk, urine, saliva, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures FX in less than 4 hours. A monoclonal antibody specific for FX has been pre-coated onto a 96-well microplate with removable strips. FX in standards and samples is sandwiched by the immobilized antibody and the peroxidase conjugated polyclonal antibody specific for FX. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated antibody (50x) 1 x 140µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Factor X Microplate (12 strips of 8 wells) 1 unit Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 1 x 30ml
- Research Areas
- SwissProt: P56693 Human
Our Abpromise guarantee covers the use of ab108832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Factor X Human ELISA kit images
Example standard curve using Factor X Human ELISA Kit ab108832. A standard curve should be generated each time the assay is performed.
References for Factor X Human ELISA kit (ab108832)
ab108832 has not yet been referenced specifically in any publications.