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Overview

  • Product nameFactor X Human ELISA kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    = 1.5 ng/ml
  • Range
    1.563 ng/ml - 100 ng/ml
  • Recovery

    101 %

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Factor X is a plasma serine protease zymogen involved in the blood coagulation cascade. Factor X is purified from plasma as a two-chain protein consisting of a 45 kDa heavy chain and a 17 kDa light chain. The Factor X heavy chain is cleaved during coagulation by several different proteases including the intrinsic Xase complex, the Factor X-activating enzyme from Russell’s viper venom (RVV) and trypsin, and also by extrinsic (tissue factor/factor VIIa) pathway to give an active enzyme FXa. FXa as the activator of prothrombin occupies a central position linking the two blood coagulation pathways.

    ab108832 Factor X Human ELISA (Enzyme-Linked Immunosorbent Assay) is designed for detection of human factor X in plasma, serum, milk, urine, saliva, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures FX in less than 4 hours. A monoclonal antibody specific for FX has been pre-coated onto a 96-well microplate with removable strips. FX in standards and samples is sandwiched by the immobilized antibody and the peroxidase conjugated polyclonal antibody specific for FX. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsSandwich ELISA more details

Properties

  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108832 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Factor X Human ELISA kit images

      • ELISA - Factor X Human ELISA kit- 1 x 96 well plate (ab108832)
        ELISA - Factor X Human ELISA kit- 1 x 96 well plate (ab108832)
        Example standard curve using Factor X Human ELISA Kit ab108832. A standard curve should be generated each time the assay is performed.

      Protocols

      References for Factor X Human ELISA kit (ab108832)

      ab108832 has not yet been referenced specifically in any publications.

      Publishing research using ab108832 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab108832 Submit a Question for ab108832

      Displaying 1 - 3 of 3 results for Abreviews and Q&A

      10
      Votes: Highest First

      Dear Sirs, I am interested in buying a Factor X Human ELISA kit (ab108832), but I need to know Which is the epitope recognized by the antibody? In other words, Does the kit recognizes the inactive and active FX (FX and FXa)? Many thanks for your help.

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      Thank you for contacting us and your interest in our products.

      The Factor X Human ELISA kit, ab108832 enables both FX and FXa to detected in a range of biological samples.

      I hope this information has been helpful. If you have any further...

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      Hello,



      I have a question about ab108832 and I would appreciate a quick response, since I will perform the assay tomorrow.



      In your “Instructions for Use” it states in the assay summary that the biot...

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      Thank you for alerting us to this mistake in the protocol.

      I can confirm that the biotinylated antibody (page 10, step 5) should be incubated for 1 hour.

      The protocol will be up-dated as soon as possible.

      We wish you good luc...

      Read More

      Dear sir, madam,



      Could you please let me know how long the fX Elisa Kits can be used? In other words, if I order an Elisa Kit now, what is the expiry date then?

      Ordering time is 1-2 weeks, as I understand?

      Read More

      Thank you for your inquiry.

      ab108832 may be storedfor up tosix monthat 2 to 8°C or -20°C from the date of shipment.

      Thiskits as all our products is covered by our Abpromiseguarantee for six month afterdate of purchase:
      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"