Overview

  • Product nameFactor X Human ELISA kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 1.5 ng/ml
  • Range
    1.563 ng/ml - 100 ng/ml
  • Recovery

    97.5 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Factor X Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor X concentrations in plasma, serum, milk, urine, saliva and cell culture supernatants.

    A Factor X specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor X specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor X captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Factor X Human ELISA kit images

  • Representative Standard Curve using ab108832

Protocols

References for Factor X Human ELISA kit (ab108832)

ab108832 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and your interest in our products.

The Factor X Human ELISA kit, ab108832 enables both FX and FXa to detected in a range of biological samples.

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Thank you for alerting us to this mistake in the protocol.

I can confirm that the biotinylated antibody (page 10, step 5) should be incubated for 1 hour.

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ab108832 may be storedfor up tosix monthat 2 to 8°C or -20°C from the date of shipment.

Thiskits as all our products is covered by our Abpromiseguarantee for six month afterdate of purchase:
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"